Effects of Microplastics on Antibiotic Resistance Genes in Estuarine Sediments

被引：15

作者：

Huang F.-Y. ^{[1
,2
]}

Yang K. ^{[1
,2
]}

Zhang Z.-X. ^{[1
]}

Su J.-Q. ^{[1
]}

Zhu Y.-G. ^{[1
]}

Zhang X. ^{[1
]}

机构：

[1] Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen

[2] University of Chinese Academy of Sciences, Beijing

来源：

Huanjing Kexue/Environmental Science | 2019年 / 40卷 / 05期

关键词：

Antibiotic; Antibiotic resistance genes(ARGs); High-throughput qPCR; Microplastics; Sediments;

D O I：

10.13227/j.hjkx.201810108

中图分类号：

学科分类号：

摘要：

Microplastics and antibiotic resistance genes (ARGs) are emerging pollutants/contaminants, and are also the research hotspots concerning environmental health in the past few years. To explore the effects of microplastics on ARGs in estuarine sediment, three different microplastics were added to microcosm incubation experiments of sediments. Then, we investigated the persistence, abundance, diversity, and shifts of the ARGs in estuarine sediments by high-throughput quantitative polymerase chain reaction (PCR). The results showed that the microplastics significantly changed the structure of ARGs in the sediments. PVC and PE, which are hard to degrade, had significant effects on the structures and types of ARGs. However, the PVA, which is soluble, reduced the types and persistence of ARGs significantly. The abundance of ARGs in S_PVC, S_PE, and S_PVA were 4.1×109, 8.1×109, and 2.0×109 copies•g-1, respectively. The abundance of ARGs in sediments with added PE almost increased by one order of magnitude, implying that microplastics could significantly increase the abundance of ARGs in sediments. Furthermore, OLS regression analysis showed that ARGs are significantly correlated with transposon and integron, suggesting that mobile genetic elements (MGEs) may promote the transfer and dissemination of ARGs. © 2019, Science Press. All right reserved.

引用

页码：2234 / 2239

页数：5

共 29 条

[1] Browne M.A., Crump P., Niven S.J., Et al., Accumulation of microplastic on shorelines woldwide: sources and sinks, Environmental Science & Technology, 45, 21, pp. 9175-9179, (2011)
[2] Barnes D.K.A., Galgani F., Thompson R.C., Et al., Accumulation and fragmentation of plastic debris in global environments, Philosophical Transactionsof the Royal Society B: Biological Sciences, 364, 1526, pp. 1985-1998, (2009)
[3] Ivar do Sul J.A., Costa M.F., The present and future of microplastic pollution in the marine environment, Environmental Pollution, 185, pp. 352-364, (2014)
[4] Wang T., Hu X.G., Zhou Q.X., The research progress in migration, distribution, biological effects and analytical methods of microplastics, Chinese Science Bulletin, 63, 4, pp. 385-395, (2018)
[5] Murphy F., Ewins C., Carbonnier F., Et al., Wastewater treatment works (WwTW) as a source of microplastics in the aquatic environment, Environmental Science & Technology, 50, 11, pp. 5800-5808, (2016)
[6] Carr S.A., Liu J., Tesoro A.G., Transport and fate of microplastic particles in wastewater treatment plants, Water Research, 91, pp. 174-182, (2016)
[7] Zhu X.T., Yi J., Qiang L.Y., Et al., Distribution and settlement of microplastics in the surface sediment of Yangtze Estuary, Environmental Science, 39, 5, pp. 2067-2074, (2018)
[8] Cole M., Lindeque P., Halsband C., Et al., Microplastics as contaminants in the marine environment: a review, Marine Pollution Bulletin, 62, 12, pp. 2588-2597, (2011)
[9] Zhang D.D., Guo Y.P., Ren H.Y., Et al., Characteristics of antibiotic resistance genes in downstream areas of the aojiang river, Fujian province, Environmental Science, 39, 6, pp. 2600-2606, (2018)
[10] Zhu Y.G., Johnson T.A., Su J.Q., Et al., Diverse and abundant antibiotic resistance genes in Chinese swine farms, Proceedings of the National Academy of Sciences of the United States of America, 110, 9, pp. 3435-3440, (2013)

← 1 2 3 →