Doxorubicin-loaded Polymeric Biotin-PEG-SeSe-PBLA Micelles with surface Binding of Biotin-Mediated Cancer Cell Targeting and Redox-Responsive Drug release for enhanced anticancer efficacy

被引：1

作者：

Gebrie, Hailemichael Tegenu ^{[1
,4
]}

Thankachan, Darieo ^{[5
]}

Tsai, Hsieh-Chih ^{[1
,2
,3
]}

Lai, Juin-Yih ^{[1
,2
,3
]}

Chang, Hao-Ming ^{[6
]}

Wu, Szu-Yuan ^{[7
,8
,9
,10
,11
,12
,13
]}

机构：

[1] Natl Taiwan Univ Sci & Technol, Grad Inst Appl Sci & Technol, Taipei 106, Taiwan

[2] Natl Taiwan Univ Sci & Technol, Adv Membrane Mat Ctr, Taipei 106, Taiwan

[3] Chung Yuan Christian Univ, R&D Ctr Membrane Technol, Taoyuan 320, Taiwan

[4] Univ Gondar, Coll Nat & Computat Sci, Dept Biol, POB 196, Gondar, Ethiopia

[5] Natl Taiwan Univ Sci & Technol, Dept Mat Sci & Engn, Taipei 106, Taiwan

[6] Triserv Gen Hosp, Natl Def Med Ctr, Div Gen Surg, Taipei, Taiwan

[7] Lotung Poh Ai Hosp, Lo Hsu Med Fdn, Dept Med, Div Radiat Oncol, Yilan, Taiwan

[8] Asia Univ, Coll Hlth Sci, Dept Hlth & Nutr Biotechnol, Taichung, Taiwan

[9] Lotung Poh Ai Hosp, Lo Hsu Med Fdn, Big Data Ctr, Yilan, Taiwan

[10] Asia Univ, Coll Med & Hlth Sci, Dept Healthcare Adm, Taichung, Taiwan

[11] Fu Jen Catholic Univ, Coll Management, Grad Inst Business Adm, Taipei, Taiwan

[12] Fu Jen Catholic Univ, Artificial Intelligence Dev Ctr, Taipei, Taiwan

[13] Taipei Med Univ, Wan Fang Hosp, Ctr Reg Anesthesia & Pain Med, Taipei, Taiwan

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2024年 / 241卷

关键词：

Diselenide bond; Biotin; Micelles; SMVT; Stimuli-sensitive polymers; DELIVERY; NANOPARTICLES; GLYCOL); SYSTEM;

D O I：

10.1016/j.colsurfb.2024.114028

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Biotin receptors are overexpressed in various cancer cell types, essential in tumor development, metabolism, and metastasis. Chemotherapeutic agents may be more effective and have fewer adverse effects if they specifically target the biotin receptors on cancer cells. Polymeric micelles (PMs) with nanoscale size via the EPR effect to accumulate near tumor tissue. We utilized the solvent exchange technique to crate polymeric Biotin-PEG-SeSePBLA micelles. This underwent self-assembly to create uniformly dispersed PMs with a hydrodynamic diameter of 81.54 +/- 0.23 nm. The resulting PMs characterized by 1HNMR, 13CNMR, FTIR, and Raman spectroscopy. PMs exhibited a high efficacy of Doxorubicin encapsulation (EE) and loading content (DLC), with values of 5.93 wt% and 74.32 %, respectively. DOX@Biotin-PEG-SeSe-PBLA micelles showed optimal DOX release, around 89 % and 74 % in 10 mM glutathione and 0.1 % H2O2, respectively, within 72 hours, in the simulated cancer redox pool. Fascinatingly, the blank Biotin-PEG-SeSe-PBLA micelles did not affect the HaCaT or HeLa cell lines; approximately 85 % of the cells were metabolically active. Contrarily, at a 5 mu g/ml concentration, DOX@Biotin-PEGSeSe-PBLA specifically inhibited the proliferation of roughly 76 % of HeLa cells and 11 % of HaCaT cells. The fluorescence microscopy results demonstrated that biotin-decorated micelles were more successfully internalized by HeLa cells, which overexpress the biotin receptor, than by non-targeted micelles in vitro. In summary, the diselenide-linked Biotin-PEGSeSe-PBLA formed smart PMs that could offer DOX specific to cancer cells with precision and are physiologically durable.

引用

页数：9