Potential application of melatonin in reducing boron toxicity in rice seedlings through improved growth, cell wall composition, proline, and defense mechanisms

被引：9
作者：
Li X. [1 ]
Kamran M. [2 ]
Saleem M.H. [3 ]
Al-Ghamdi A.A. [4 ]
Al-Hemaid F.M. [4 ]
Elshikh M.S. [4 ]
Zhao S. [1 ]
Riaz M. [1 ]
机构：
[1] Guangdong Engineering and Technology Center for Environmental Pollution Prevention and Control in Agricultural Producing Areas, College of Resources and Environment, Zhongkai University of Agriculture and Engineering
[2] School of Agriculture, Food, and Wine, The University of Adelaide, 5005, SA
[3] Office of Academic Research, Office of VP for Research & Graduate Studies, Qatar University, Doha
[4] Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh
来源：
Chemosphere | 2023年 / 335卷
关键词：
Antioxidant capacity; Boron toxicity; Cell wall; Melatonin; Proline metabolism; Rice plants;
D O I：
10.1016/j.chemosphere.2023.139068
中图分类号：
学科分类号：
摘要：
Melatonin (MT) has been demonstrated to provide defense against both biotic and abiotic stressors. Boron toxicity (BT) can significantly limit the growth and production of plants. However, few studies have been conducted on whether MT is effective in attenuating B toxicity in different plants. In order to evaluate the efficacy of exogenous MT treatment in reducing the negative impact of BT on rice seedlings, this study examined the influence of MT on growth, antioxidant capacity, cell wall composition, and proline metabolism in rice seedlings under hydroponics. Four treatments were established: MT (50 μM), MT + BT (50 μM MT + 800 μM B), BT (800 μM), and CK (control) in a completely randomized design. The results indicate that BT had a significant detrimental effect on the shoot length, root length, and root and shoot fresh weights of rice seedlings by 11.96%, 27.77%, 25.69%, and 18.67%, respectively as compared to the control treatment. However, exogenous MT application increased these parameters and reduced B accumulation in aboveground parts (14.05%) of the plant. Exogenous MT also increased the endogenous melatonin content and antioxidant enzyme activities (64.45%, 71.61%, 237.64%, and 55.42% increase in superoxide dismutase, ascorbate peroxidase, and peroxidase activities, respectively), while decreasing reactive oxygen species levels and oxidized forms of glutathione and ascorbic acid. Additionally, MT enhanced the biosynthesis of proline by decreasing proline dehydrogenase (ProDH) and increasing the GSH (glutathione) and ASA (ascorbic acid) contents. Exogenous MT also increased cell wall components that can increase B adsorption to the cell wall. Overall, these findings suggest that MT application can be a potential solution for strengthening the stress tolerance of rice seedlings, particularly under conditions of B toxicity. In regions where soil contains high levels of boron, the use of MT could enhance rice crop yields and quality. © 2023 Elsevier Ltd
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