In Vivo Treatment Efficacy of Essential Oil Isolated from Seeds of Momordica charantia in Streptozotocin-Induced Diabetes Mellitus

被引：3
作者：
Mariammal B.G.V. [1 ]
Devarajan D.W. [2 ]
Jerrin R. [1 ]
Viswanathan S. [1 ]
Siddikuzzaman [3 ]
Gopal R. [4 ]
机构：
[1] Department of Biotechnology, Karunya Institute of Technology and Sciences, Karunya Nagar, Tamil Nadu, Coimbatore
[2] School of Science, Arts, Media and Management (SSAM-M), Karunya Institute of Technology and Sciences, Karunya Nagar, Tamil Nadu, Coimbatore
[3] International Institute of Innovation & Technology (IIIT), Action Area 1D, West Bengal, Newtown, Kolkata
[4] Department of Zoology, Annamalai University, Annamalai Nagar, Tamil Nadu, Chidambaram
来源：
Recent Patents on Biotechnology | 2021年 / 15卷 / 04期
关键词：
Cucurbitaceae; Diabetes mellitus; Essential oil; GC-MS; Momordica charantia L; Phytochemicals; Streptozo-tocin;
D O I：
10.2174/1872208315666210910092105
中图分类号：
学科分类号：
摘要：
Background: All parts of Momordica charantia L. have potential hypoglycemic properties in reversing the metabolic disorder of diabetes mellitus. However, there exists a need for preparing an effective and safer formulation of active phytochemicals. We have also reviewed and analyzed certain patents on such preparatory methods for Momordica charantia L. formulations. Objective: This study aimed to isolate essential oil from the seeds of Momordica charantia L., analyze its phytochemicals, and study their anti-diabetic effects. Methods: The essential oil was isolated by the hydrodistillation method and analyzed for phytochemicals by GC-MS. Furthermore, its acute toxicity was tested in rats. Anti-diabetic effects were evaluated in Streptozotocin-induced diabetic rats with 17.5 and 55 mg/kg b.wt of essential oil by evaluating blood glucose, serum lipid profile, liver glycogen, protein, and other serum markers such as ALT, AST, ALP, urea, and creatinine. The histologic changes in the liver, pan-creas, and kidney were evaluated using Haematoxylin and Eosin staining. Results: The phytochemicals having hypoglycaemic and insulin induction poten-cy were identified in the GC-MS analysis. A highly significant (p≤0.01; p≤0.001) reduction in blood glucose was observed from 17.5 mg/kg and 55 mg/kg essential oil treatments, respectively. Diabetes-associated metabolic altera-tions (p≤0.001) observed in diabetic control rats such as lipid profile, enzymes, glycogen, protein, urea, and creatinine were normalized upon treatment with essential oil. Moreover, the histologic changes in vital organs reversed in treated rats. Conclusion: The essential oil of Momordica charantia L. seed has promising po-tency to normalize the metabolic changes of type II diabetes mellitus. © 2021 Bentham Science Publishers.
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页码：316 / 331
页数：15
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