Screening, Identification and Action Mechanism of T-2 Toxin-Degrading Strains

被引：0
作者：
Ma Y. [1 ,2 ]
Sun C. [2 ]
Wang J. [2 ]
Du W. [2 ]
Liu H. [2 ]
Zhou W. [1 ]
Zhao Y. [2 ]
机构：
[1] College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha
[2] Academy of National Food and Strategic Reserves Administration, Beijing
来源：
Shipin Kexue/Food Science | 2023年 / 44卷 / 22期
关键词：
biodegradation; degradation mechanism; identification; metabolites; screening; T-2; toxin;
D O I：
10.7506/spkx1002-6630-20221111-126
中图分类号：
学科分类号：
摘要：
The purpose of this study was to obtain microbial strains capable of high-efficiency degradation of T-2 toxin and to investigate their mechanism of action. T-2 toxin-degrading microorganisms were isolated from wheat and identified and their degradation characteristics were explored; their metabolites and mechanism of action were analyzed by ultrahigh performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). The results showed that two efficient T-2 toxin degrading strains, AFJ-2 and AFJ-3, were screened from 50 wheat samples and identified respectively as Curtobacterium sp. and Bacillus sp. by morphological observation and 16S rDNA sequence analysis. T-2 toxin at 5 μg/mL was completely degraded within 7 and 12 h by strains AFJ-2 and AFJ-3, respectively, and intracellular enzymes from them could significantly degrade T-2 toxin (P < 0.05) without adsorption. Strain AFJ-2 was able to convert T-2 toxin to HT-2 toxin and T-2 triol, while strain AFJ-3 could degrade T-2 toxin to produce neosolaniol (NEO). Based on the predicted degradation sites, it was hypothesized that the two strains could work together to degrade T-2 toxin to produce a new product, 4-deacetyl-NEO. The research results enrich the strain resource bank for the biodegradation of T-2 toxin, and provide a reference for the separation and purification of T-2 toxin-degrading enzymes and the excavation of functional genes. © 2023 Chinese Chamber of Commerce. All rights reserved.
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页码：173 / 182
页数：9
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