Development of an odorant sensor with a cell-free synthesized olfactory receptor and a graphene field-effect transistor

被引：0

作者：

Yoshii T. ^{[1
]}

Takayama I. ^{[2
]}

Fukutani Y. ^{[2
]}

Ikuta T. ^{[1
]}

Maehashi K. ^{[1
]}

Yohda M. ^{[2
]}

机构：

[1] Department of Advanced Applied Physics, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho,Koganei, Tokyo

[2] Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho,Koganei, Tokyo

来源：

Analytical Sciences | 2022年 / 38卷

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

Chemical sensor; Graphene; In vitro expression; Mini-g protein; Olfactory receptor;

D O I：

10.1007/S44211-022-00073-Y

中图分类号：

学科分类号：

摘要：

Animals sense odorants using olfactory receptors. Many trials have been conducted to develop artificial odorant sensors using olfactory receptors. However, the development has been hindered by the difficulty in obtaining olfactory receptors. In this study, we expressed an olfactory receptor, cOR52, using a wheat germ cell-free synthesis system. The functionality of the expressed cOR52 was confirmed by ligand concentration-dependent interactions with the mini-G protein. The expressed cOR52 was immobilized on a graphene field-effect transistor. The cOR52-modified graphene field-effect transistor exhibited a ligand-specific response between 100 nM and 100 μM. This approach seems to be applicable for other olfactory receptors. Therefore, it will be possible to develop an odorant sensor equipped with various olfactory receptors by this method. © The Author(s), under exclusive licence to The Japan Society for Analytical Chemistry 2022

引用

页码：241 / 245

页数：4

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