Determination of Benzo[a]pyrene in Smoked Sturgeon (Acipenser sinensis) Using Molecular-imprinted Solid-phase Extraction Liquid Chromatography Tandem Mass Spectrometry Method

被引：0
|
作者：
Lin Y. [1 ]
Li S. [2 ]
Cui Y. [1 ]
Dai Z. [1 ,3 ]
Chen K. [1 ,3 ]
Wang Y. [2 ]
Shen Q. [1 ,3 ]
机构：
[1] Institute of Seafood, Zhejiang Gongshang University, Hangzhou
[2] Aquatic Products Quality Inspection Center of Zhejiang Province, Hangzhou
[3] Key Laboratory of Aquatic Products Processing of Zhejiang Province, Hangzhou
来源：
Journal of Chinese Institute of Food Science and Technology | 2020年 / 20卷 / 03期
关键词：
Benzo[a]pyrene; Liquid chromatography; Mass spectrometry; Molecular-imprinted solid-phase extraction; Smoked sturgeon;
D O I：
10.16429/j.1009-7848.2020.03.031
中图分类号：
学科分类号：
摘要：
The current normal method for the determination of benzo(a)pyrene is not specific, and its sensitivity is not very good. The 3, 4-benzo(a)pyrene(3, 4-BaP) is an organic compound with the formula C20H12. 3, 4-BaP is a harmful compound, because it generates carcinogenic and mutagenic metabolites which can intercalate into DNA and interference with transcription. In this study, the molecular imprinted sorbent was synthesized and packed into a solid-phase extraction (SPE) cartridge for the enrichment of 3, 4-BaP in smoked sturgeon (Acipenser sinensis) bought from local market. An analytical method was also developed for accurate determination of 3, 4-BaP on the basis of ultra-performance liquid chromatography tandem mass spectrometry. The molecular imprinted sorbents are dispersed spherical particles, with diameter from 20 to 90 μm. It was successfully applied in the enriching of 3, 4-BaP, and its performance was compared with Sep-pak, HLB, and Florisil SPE. The method was validated, and the results showed that the calibration curve is linear with R2 = 0.9998. The limit of detection (LoD) and limit of quantitation (LoQ) are 0.98 and 3.16 μg/kg, respectively. The RSDs of intra-day and inter-day accuracy are 3.26%-4.77% and 3.78%-5.26%, respectively. The recovery ranged from 89.7% to 95.3%, with RSD 2.89%-3.44%. Afterwards, the method was successfully applied to determine the 3, 4-BaP in 50 batches of smoked sturgeon. The results indicated that 12 positive samples were found. The content of 3, 4-BaP in the worst sample reached 21.9 μg/kg. The contents of 3, 4-BaP in the other samples are lower than 3.58 μg/kg. In conclusion, this method is good at determining 3, 4-BaP in smoked sturgeon. The relevant data could be used as reference for the functional departments for ensuring the food safety for consumers. © 2020, Editorial Office of Journal of CIFST. All right reserved.
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页码：251 / 257
页数：6
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共 13 条
  • [1] Hu J., Zhang Z., Wei Q., Et al., Malformations of the endangered Chinese sturgeon, Acipenser sinensis, and its causal agent, Proceedings of the National Academy of Sciences, 106, 23, pp. 9339-9344, (2009)
  • [2] Hong C.H., Lee C.H., Yu H.S., Et al., Benzopyrene, a major polyaromatic hydrocarbon in smoke fume, mobilizes Langerhans cells and polarizes Th2/17 responses in epicutaneous protein sensitization through the aryl hydrocarbon receptor, International Immunopharmacology, 36, pp. 111-117, (2016)
  • [3] Simko P., Changes of benzo (a) pyrene contents in smoked fish during storage, Food Chemistry, 40, 3, pp. 293-300, (1991)
  • [4] Shao Y., Wang J., Chen X., Et al., The consolidation of food contaminants standards in China, Food Control, 43, pp. 213-216, (2014)
  • [5] Menezes H.C., Paulo B.P., Paiva M.J.N., Et al., Determination of polycyclic aromatic hydrocarbons in artisanal cachaça by DI-CF-SPME-GC/MS, Microchemical Journal, 118, pp. 272-277, (2015)
  • [6] Gazioglu I., Tekkeli S.E.K., Development and validation of a HPLC method for the determination of benzo (a) pyrene in human breast milk, Food Science and Biotechnology, 26, 2, pp. 319-322, (2017)
  • [7] Jiang H., Gelhaus S.L., Mangal D., Et al., Metabolism of benzo [a] pyrene in human bronchoalveolar H358 cells using liquid chromatography-mass spectrometry, Chemical Research in Toxicology, 20, 9, pp. 1331-1341, (2007)
  • [8] Pschenitza M., Hackenberg R., Niessner R., Et al., Analysis of benzo[a] pyrene in vegetable oils using molecularly imprinted solid phase extraction(MISPE) coupled with enzyme-linked immunosorbent assay(ELISA), Sensors, 14, 6, pp. 9720-9737, (2014)
  • [9] Shen Q., Dai Z., Lu Y., Rapid determination of caffeoylquinic acid derivatives in Cynara scolymus L. By Ultra-Fast Liquid Chromatography/tandem Mass Spectrometry Based on a Fused Core C18 column, Journal of Separation Science, 33, 20, pp. 3152-3158, (2010)
  • [10] Jin R., Li L., Guo L., Et al., A graphene tip coupled with liquid chromatography tandem mass spectrometry for the determination of four synthetic adulterants in slimming supplements, Food Chemistry, 224, pp. 329-334, (2017)
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