Enhancing the Neuroprotection Potential of Edaravone in Transient Global Ischemia Treatment with Glutathione- (GSH-) Conjugated Poly(methacrylic acid) Nanogel as a Promising Carrier for Targeted Brain Drug Delivery

被引：6

作者：

Mozafari F. ^{[1
,2
]}

Rashidzadeh H. ^{[3
,4
]}

Bijani S. ^{[1
]}

Zare-Molaei F. ^{[1
]}

Islambulchilar Z. ^{[5
]}

Danafar H. ^{[1
]}

Kalantari-Hesari A. ^{[6
]}

Ramazani A. ^{[3
,4
]}

Hosseini M.-J. ^{[1
]}

机构：

[1] Zanjan Applied Pharmacology Research Center, Zanjan University of Medical Sciences, Zanjan

[2] Department of Pharmacology and Toxicology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan

[3] Pharmaceutical Biotechnology Research Center, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan

[4] Pharmaceutical Biomaterials Department, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan

[5] Department of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz

[6] Department of Pathobiology, Faculty of Veterinary Sciences, Bu-Ali Sina University, Hamedan

来源：

Oxidative Medicine and Cellular Longevity | 2023年 / 2023卷

关键词：

Antioxidants - Biochemistry - Controlled drug delivery - Drug products - Morphology - Nanostructured materials - Peptides - Targeted drug delivery;

D O I：

10.1155/2023/7643280

中图分类号：

学科分类号：

摘要：

Ischemic stroke is the most common among various stroke types and the second leading cause of death, worldwide. Edaravone (EDV) is one of the cardinal antioxidants that is capable of scavenging reactive oxygen species, especially hydroxyl molecules, and has been already used for ischemic stroke treatment. However, poor water solubility, low stability, and bioavailability in aqueous media are major EDV drawbacks. Thus, to overcome the aforementioned drawbacks, nanogel was exploited as a drug carrier of EDV. Furthermore, decorating the nanogel surface with glutathione as targeting ligands would potentiate the therapeutic efficacy. Nanovehicle characterization was assessed with various analytical techniques. Size (199 nm, hydrodynamic diameter) and zeta potential (-25 mV) of optimum formulation were assessed. The outcome demonstrated a diameter of around 100 nm, sphere shape, and homogenous morphology. Encapsulation efficiency and drug loading were determined to be 99.9% and 37.5%, respectively. In vitro drug release profile depicted a sustained release process. EDV and glutathione presence in one vehicle simultaneously made the possibility of antioxidant effects on the brain in specific doses, which resulted in elevated spatial memory and learning along with cognitive function in Wistar rats. In addition, significantly lower MDA and PCO and higher levels of neural GSH and antioxidant levels were observed, while histopathological improvement was approved. The developed nanogel can be a suited vehicle for drug delivery of EDV to the brain and improve ischemia-induced oxidative stress cell damage. © 2023 Faezeh Mozafari et al.

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