Construction and evaluation of gene delivery system based on polyacylthiourea

被引：0
作者：
Wang Y. [1 ]
Zhou Z.-X. [1 ]
Shen Y.-Q. [1 ]
机构：
[1] College of Chemical and Biological Engineering, Zhejiang University, Hangzhou
来源：
Zhejiang Daxue Xuebao (Gongxue Ban)/Journal of Zhejiang University (Engineering Science) | 2019年 / 53卷 / 03期
关键词：
Facile synthesis; Gene delivery; Polyacylthiourea; Transfection efficiency;
D O I：
10.3785/j.issn.1008-973X.2019.03.022
中图分类号：
学科分类号：
摘要：
N, N-dimethylcysteamine modified PATU (PATU-DMCA) was synthesized through thiol-ene Michael addition reaction based on a novel dendrimer of polyacylthiourea (PATU). NMR results show that the dendrimer structure of PATU-DMCA is correct and integrated. Agarose gel electrophoresis assay and dynamic light scattering results demonstrate that PATU-DMCA of low generations with low molar ratios of N and P can package DNA to form nanoparticles with size of 60~100 nm and Zeta potential of 10~20 mV. In human cervical carcinoma cell HeLa and human lung cancer cell A549, the formed polyplexes with low molar ratios of N and P show higher transfection efficiency in serum-free medium than the classical dendrimer poly (amidoamine), while the cytotoxicity is equal to each other. The subcellular distribution study in HeLa indicate that the intracellular trafficking of the polyplexes include cell membrane adhesion, endocytosis into cell, entering into lysosome, and lysosomal escape via 'proton sponge effect' for transfection in cytoplasm and nucleus. Both PATU-DMCA and PATU itself have enormous potential for gene delivery. © 2019, Zhejiang University Press. All right reserved.
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页码：598 / 604
页数：6
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