Synthesis and Properties of A Mitochondria-targeted Coumarin-based pH Fluorescent Probe

被引：0

作者：

Chen J. ^{[1
]}

Feng Y. ^{[1
]}

Meng X.-M. ^{[1
]}

机构：

[1] School of Chemistry and Chemical Engineering, Anhui University, Hefei

来源：

Faguang Xuebao/Chinese Journal of Luminescence | 2021年 / 42卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Coumarin; Fluorescent probe; Mitochondria; PH;

D O I：

10.37188/CJL.20200363

中图分类号：

学科分类号：

摘要：

Mitochondrion is an important organelle whose functions include energy supply, signal transduction, cell differentiation, cell death, and the control of cell physiological cycle and cell growth. Any damage to mitochondria and subsequent dysfunction could lead to a range of human diseases. Mitochondrial pH directly affects its biochemical process, and the normal pH level is the basis for the normal operation of mitochondrial function. In this paper, a mitochondria-targeted pH fluorescent probe(CMPH) was synthesized by choosing 7-hydroxycoumarin as the raw material. Its optical properties, cytotoxicity and mitochondria-targeted ability were studied. The results showed that probe CMPH had a large Stokes shift, can specifically give a fluorescent response to pH, and its fluorescent intensities had a good linear relationship to pH values in the range of pH=6.5-8.2. Cytotoxicity testing indicated that probe CMPH had low cytotoxicity. Confocal fluorescence imaging demonstrated that CMPH can efficiently target mitochondria and monitor the change of mitochondrial pH. Our purpose is to provide a new imaging tool for monitoring the change of mitochondrial pH. © 2021, Science Press. All right reserved.

引用

页码：462 / 469

页数：7

共 35 条

[1]

ZHONG W X, RABINOWITZ J D, WHITE E., Mitochondria and cancer, Mol. Cell, 61, 5, pp. 667-676, (2016)

[2]

HAJNOCZKY G, CSORDAS G, YI M., Old players in a new role: mitochondria-associated membranes, VDAC, and ryanodine receptors as contributors to calcium signal propagation from endoplasmic reticulum to the mitochondria, Cell Calcium, 32, 5-6, pp. 363-377, (2002)

[3]

WANG H, LIU C, ZHAO Y X, Et al., Mitochondria regulation in ferroptosis, Eur. J. Cell Biol, 99, 1, (2020)

[4]

ZHAO Z Z, FU A L, Mitochondrial therapy: a new strategy for treating mitochondrion-associated diseases, Chin. J. Biotech, (2021)

[5]

CHAKRABARTY S, KABEKKODU S P, SINGH R P, Et al., Mitochondria in health and disease, Mitochondrion, 43, pp. 25-29, (2018)

[6]

DING W X, YIN X M., Mitophagy: mechanisms, pathophysiological roles, and analysis, Biol. Chem, 393, 7, pp. 547-564, (2012)

[7]

KHAN N A, GOVINDARAJ P, SOUMITTRA N, Et al., Leber's hereditary optic neuropathy-specific mutation m. 11778G>A exists on diverse mitochondrial haplogroups in India, Invest. Ophthalmol. Vis. Sci, 58, 10, pp. 3923-3930, (2017)

[8]

WU M Y, LI K, LIU Y H, Et al., Mitochondria-targeted ratiometric fluorescent probe for real time monitoring of pH in living cells, Biomaterials, 53, pp. 669-678, (2015)

[9]

LI X L, ZHENG J Z, LIU W J, Et al., Long-term super-resolution imaging of mitochondrial dynamics, Chin. Chem. Lett, 31, 11, pp. 2937-2940, (2020)

[10]

FANG Z J, SU Z, QIN W J, Et al., Two-photon dual-channel fluorogenic probe for in situ imaging the mitochondrial H<sub>2</sub>S/viscosity in the brain of drosophila Parkinson's disease model, Chin. Chem. Lett, 31, 11, pp. 2903-2908, (2020)

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