Growth Characteristics of Bacillus velezensis Antagonistic to Botrytis Cinerea and Its Effects on Related Defense Enzyme Activities

被引：0

作者：

Zou Q. ^{[1
,2
]}

Niu X. ^{[3
,4
]}

Liu P. ^{[1
,2
]}

Yang H. ^{[2
,5
]}

Chu M. ^{[2
,5
]}

Wang N. ^{[2
,5
]}

Lin Q. ^{[2
,5
]}

Bao H. ^{[2
,5
]}

Zhan F. ^{[2
,5
]}

Zhang Y. ^{[1
,2
]}

Wang J. ^{[1
,2
]}

Zuo C. ^{[1
,2
]}

Lou K. ^{[2
,5
]}

Shi Y. ^{[1
,2
,4
,5
]}

机构：

[1] College of Life Science and Technology, Xinjiang University, Urumqi

[2] Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi

[3] Institute of Soil and Fertilizer and Agricultural Water Conservation, Xinjiang Academy of Agricultural Sciences, Urumqi

[4] Key Laboratory of Northwest Oasis Agricultural Environment, Ministry of Agriculture and Rural Affairs, Urumqi

[5] Xinjiang Special Environmental Microbiology Laboratory, Urumqi

来源：

Science and Technology of Food Industry | 2023年 / 44卷 / 15期

关键词：

antibiotic marker; Bacillus velezensis; colonization; enzymatic activity; gray mold;

D O I：

10.13386/j.issn1002-0306.2022060270

中图分类号：

学科分类号：

摘要：

To clarify the mechanism of resistance of Bacillus velezensis TP-1 to gray mold, a marker strain TP-1R was screened for resistance to 300 μg/mL of rifampicin by an antibiotic marker method in this study. The genetic stability of the resistance of the marker strains and their antagonism to gray mold of grapes were studied, and their colonization in grapes and their effect on the activity of defence enzymes in the grapes were analysed. The results showed that the labeled strain could still grow stably in the medium containing rifampicin after 15 times of subculture, and the antagonistic effect of the labeled strain against gray mold was not significantly different from that of the original strain. The colonization amount of the labeled strain reached the peak of 4.32×106 CFU/g on the 15th day of storage (20 ℃), and was still 3.11×106 CFU/g on the 30th day of storage, indicating that strain TP-1 could be stably colonized on grapes. Inoculation of strain TP-1 fermentation broth could effectively inhibit the occurrence of gray mold and resultantly reduced the decay rate of grapes. During storage, the activity of grape defence enzymes PAL, PPO and APX increased first and then decreased, and the activity of the three enzymes in the group treated with the antagonistic bacterium TP-1 was significantly higher than CK (P<0.05). At the 15th day, the activities of PAL, PPO and APX in the antagonistic bacterium treatment were 1.23, 1.19 and 2.01 times higher than CK. The colonization of strain TP-1 on grape could enhance activities of a set of defense-related enzymes, including PAL, PPO and APX, and enhance the resistance of grape to gray mold to a certain extent. This study provided a scientific basis for revealing the mechanism involved in the biocontrol effectiveness of Bacillus velezensis TP-1. © Science and Technology of Food Industry. All rights reserved.

引用

页码：126 / 133

页数：7

共 39 条

[1] LI J., The study on biocontrol of post harvest diseases of Muscat grapes by Burkholderia contaminans B-1, (2016)
[2] NALLY M C, PESCE V M, MATURANO Y P, Et al., Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces cerevisiae yeasts isolated from viticultural environments in Argentina[J], Postharvest Biology & Technology, 64, 1, (2012)
[3] FURUYA S, MOCHIZUKI M, AOKI Y, Et al., Isolation and characterization of Bacillus subtilis KS1 for the biocontrol of grapevine fungal diseases[J], Biocontrol Science and Technology, 21, 6, (2011)
[4] LI Q, LI C, LI P, Et al., The biocontrol effect of Sporidiobolus pararoseus Y16 against postharvest diseases in table grapes caused by Aspergillus niger and the possible mechanisms involved[J], Biological Control, 113, (2017)
[5] ZHANG W, ZHOU Y, PENG Y J, Et al., Identification of grape gray mold pathogen and evaluation of inhibitory effect of natural fungicide[J]. Sino-Overseas Grapevine & Wine, 2021(4): 26−32.][6] YANG L, ZENG K, MING J. Control of blue and green mold decay of citrus fruit by Pichia membranefaciens and induction of defense responses, Entia Horticulturae, 135, pp. 120-127, (2012)
[6] CHEN Z Y., Research and application of bio-fungicide with Bacillus spp.[J], Chinese Journal of Biological Control, 31, 5, (2015)
[7] SHEN H M, LI Z N, JIA Z S, Et al., Colonization of grape leaves by endophytic Bacillus subtilis JL4 and its control of grape downy mildew, Chinese Journal of Applied Ecology, 27, 12, (2016)
[8] BACON C W, YATES I E, HINTON D M, Et al., Biological control of Fusarium moniliforme in Maize[J], Environmental Health Perspectives, 109, 2, (2001)
[9] GAO Z F, ZHAO J., Effect of lipopeptide by Bacillus velezensis ZSY-1 on tomato postharvest softening and early blight [J], Acta Agriculturae Boreali-occidentalis Sinica, 31, 1, (2022)
[10] DESOIGNIES N, SCHRAMME F, ONGENA M, Et al., Systemic resistance induced by Bacillus lipopeptides in Beta vulgaris reduces infection by the rhizomania disease vector Polymyxa betae[J], Molecular Plant Pathology, 14, 4, (2013)

← 1 2 3 4 →