Protective Effect of Ganoderma atrum Polysaccharides against Acrylamide-Induced Liver Injury in Rats

被引：2

作者：

Jiang G. ^{[1
]}

Lei A. ^{[1
]}

Yang Y. ^{[1
]}

Yu Q. ^{[1
]}

Xie J. ^{[1
]}

Chen Y. ^{[1
]}

机构：

[1] State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang

来源：

Shipin Kexue/Food Science | 2020年 / 41卷 / 01期

关键词：

Acrylamide; Ganoderma atrum polysaccharides; Liver injury; Oxidative damage;

D O I：

10.7506/spkx1002-6630-20190623-269

中图分类号：

学科分类号：

摘要：

Purpose: This study aimed to investigate the protective effect of Ganoderma atrum polysaccharides (PSG) on acrylamide (AA)-induced liver injury in rats. Methods: A total of 60 SD male rats were randomly divided into 6 groups of 10 each: normal control, model (20 mg/kg m不 of AA), positive control (N-acetyl-L-cysteine, NAC; at 200 mg/kg mb) and low-, medium and high-dose PSG treatment groups (at doses of 50, 100 and 200 mg/kg mb). The rats in the normal control group were given normal saline at the same volume as the corresponding drugs for the other groups. Starting from half an hour after the administration, the AA aqueous solution was orally administered for 30 continuous days. Pathological observation of liver tissues was performed by hematoxylin and eosin (HE) staining. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) and the concentrations of triglyceride (TG) and interleukin (IL-1β and IL-10) in serum were measured. Then, the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) and the content of MDA in liver tissue homogenate were measured. Results: AA significantly increased AST, ALT and ALP activity as well as TG and IL-1β concentration, and decreased IL-10 levels in serum (P < 0.05); significantly reduced the activity of SOD and CAT and GSH-Px and increased MDA content in liver tissue (P < 0.05); and caused pathological liver injury. PSG obviously attenuated all these negative effects. Conclusion: Ganoderma atrum polysaccharides can have a significant protective effect on acrylamide-induced liver injury. © 2020, China Food Publishing Company. All right reserved.

引用

页码：121 / 126

页数：5

共 26 条

[1]

Ghorbel I., Elwej A., Chaabene M., Et al., Effects of acrylamide graded doses on metallothioneins I and II induction and DNA fragmentation: bochemical and histomorphological changes in the liver of adult rats, Toxicology and Industrial Health, 33, 8, pp. 611-622, (2017)

[2]

Tareke E., Rydberg P., Karlsson P., Et al., Analysis of acrylamide, a carcinogen formed in heated foodstuffs, Journal of Agricultural and Food Chemistry, 50, 17, pp. 4998-5006, (2002)

[3]

Zhang L., Xu Y., Li Y., Et al., Protective property of mulberry digest against oxidative stress: a potential approach to ameliorate dietary acrylamide-induced cytotoxicity, Food Chemistry, 230, pp. 306-315, (2017)

[4]

Friedman M., Acrylamide: inhibition of formation in processed food and mitigation of toxicity in cells, animals, and humans, Food & Function, 6, 6, pp. 1752-1772, (2015)

[5]

Seydi E., Rajabi M., Salimi A., Et al., Involvement of mitochondrial mediated caspase-3 activation and lysosomal labilization in acrylamide induced liver toxicity, Toxicological & Environmental Chemistry, 97, 5, pp. 563-575, (2015)

[6]

Watzek N., Scherbl D., Schug M., Et al., Toxicokinetics of acrylamide in primary rat hepatocytes: coupling to glutathione is faster than conversion to glycidamide, Archives of Toxicology, 87, 8, pp. 1545-1556, (2013)

[7]

Zhang X., Jiang L., Geng C., Et al., Inhibition of acrylamide genotoxicity in human liver-derived HepG2 cells by the antioxidant hydroxytyrosol, Chemico-Biological Interactions, 176, 2-3, pp. 173-178, (2008)

[8]

Gedik S., Erdemli M.E., Gul M., Et al., Hepatoprotective effects of crocin on biochemical and histopathological alterations following acrylamide-induced liver injury in Wistar rats, Biomedicine & Pharmacotherapy, 95, pp. 764-770, (2017)

[9]

Shrivastava S., Uthra C., Reshi M., Et al., Protective effect of hesperetin against acrylamide induced acute toxicity in rats, Indian Journal of Experimental Biology, 56, 3, pp. 164-170, (2018)

[10]

Alturfan A.A., Tozan-Beceren A., Sehirli A.O., Et al., Resveratrol ameliorates oxidative DNA damage and protects against acrylamide-induced oxidative stress in rats, Molecular Biology Reports, 39, 4, pp. 4589-4596, (2012)

← 1 2 3 →