Chain-locked precursor ion scanning based HPLC–MS/MS for in-depth molecular analysis of lipase-catalyzed transesterification of structured phospholipids containing ω-3 fatty acyl chains

被引：5

作者：

Zhang M. ^{[1
,2
]}

Wang P. ^{[3
]}

Jin D. ^{[2
]}

Jian S. ^{[2
]}

Wu J. ^{[2
]}

Huang M. ^{[2
]}

Xie H. ^{[2
]}

Zhao Q. ^{[3
]}

Yang H. ^{[5
]}

Luo P. ^{[4
]}

Yuan H. ^{[1
]}

Xue J. ^{[2
]}

Shen Q. ^{[1
,2
]}

机构：

[1] First People's Hospital of Linping District, Hangzhou

[2] Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou

[3] Zhoushan Institute of Food & Drug Control, Zhoushan

[4] State Key Laboratories for Quality Research in Chinese Medicines, Faculty of Pharmacy, Macau University of Science and Technology, Macau

[5] Zhejiang Marine Development Research Institute, Zhoushan

来源：

Food Chemistry | 2023年 / 399卷

基金：

中国国家自然科学基金;

关键词：

Lipase-catalysis; Lipidomics; Phospholipase; Precursor ion scanning; Transesterification;

D O I：

10.1016/j.foodchem.2022.133982

中图分类号：

学科分类号：

摘要：

Lipase-catalyzed transesterification of structured phospholipids (sPLs) is a hot topic, but the structural variation of the fatty acyl chains in intact phospholipids at the molecular level remains unclear to date. The present study explored the detailed characteristics of synthesized phospholipids through high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) in precursor ion scan mode. The optimal conditions were in-depth inspected and determined for the reaction system, including phospholipase A1 as catalyst, 15% lipase loading, and 1% water content. The sPLs enriched with EPA/DHA were structurally and quantitatively characterized by focusing on the fragments of m/z 301.6 (eicosapentaenoic acid, EPA) and m/z 327.6 (docosahexaenoic acid, DHA), and the results were statistically analyzed using partial least squares discriminant analysis and clustered heatmap hierarchical clustering analysis. PC 38:6 (18:1/20:5), PC 38:7 (18:2/20:5), PC o-40:6 (o-18:0/22:6), and PE 40:8 (18:2/22:6) etc. were revealed as the main variables that were active in the reaction. © 2022 Elsevier Ltd

引用

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