[Objective] To solvethe problem ofthe uneven distribution of waterresources, the country has built many water infrastructures, with long engineering pipelines, strong water conveyance capacity, and high safety and reliability. The Dianzhong water diversion projectisthelargest waterinfrastructure project under constructionin China, which caneffectively alleviate the shortage of water resources in the central area of Yunnan Province, and ensure sustainable economic and social development after its completion. The aqueduct is one of the important water diversion structures in the project, which is generally used to cross special terrains such as rivers and valleys. The aqueduct construction of the project faces many challenges, such as high seismic intensity, multiple active faults, and complex geological conditions. Ensuring the structural safety of the aqueduct is the key prerequisite for guaranteeing the continuous water diversion function. [Methods] Extensive theoretical analysis, model tests, and numerical simulation studies have been conducted on the structural safety of the aqueduct in the Dianzhong water diversion project. The researches include various aspects, such as the design and optimization of the aqueduct structure, water-aqueduct interaction, soil-structure interaction, complex geological conditions, and the seismic performance and isolation performance of the aqueduct, etc. . [Results] Specifically, to improve the cracking resistance ability of the aqueduct, the influence of temperature gradient and layout of prestressed steel strands was discussed, and the design recommendation was suggested. In order to better illustrate the water-aqueduct interaction and the soil-structure interaction, some design methods were successively proposed, and the interaction mechanism was further explored. The impact of complex geological conditions was considered when conducting the dynamic analysis to obtain a more realistic earthquake response to the aqueduct. To effectively control the seismic response of aqueducts, many types of energy dissipation devices and isolation bearings were proposed, and the seismic performance and isolation performance of the aqueduct with new devices were investigated. [Conclusions] Based on the actual practice of the Dianzhong water diversion project, this paper summarizes the relevant research results and the future research trends of aqueducts, aiming to provide a reference for the project construction. Research shows that a key to accurately analyzing the seismic response of the aqueduct is to simulate the interaction between water and aqueduct, and soil and structure. The analysis technique considering the water-aqueduct interaction and the soil-structure interaction is formed, which provides support for numerical analysis of the aqueduct. On this basis, the seismic performance and isolation performance of aqueducts under high seismic intensity and complex geological conditions are deeply investigated. The impact mechanism of terrain differences on structural seismic performance, such as V-shaped river valleys, is analyzed. The seismic isolation mechanisms of different seismic isolation measures are clarified. With the continuous development of society, the demand for aqueduct structures in terms of water conveyance capacity and spanning capacity increases. It is significant to further ensure the safety and sustainable water transportation of aqueducts under high seismic intensity and complex geological conditions. Utilizing the shaking of water in the aqueduct to achieve seismic reduction effects, and developing new seismic isolation devices and anti-seepage waterproofs are important development directions in the future. © 2024 Tsinghua University. All rights reserved.