A composite enzyme derived from papain and chymotrypsin reduces the Allergenicity of Cow's Milk allergen casein by targeting T and B cell epitopes

被引：4

作者：

Xiong, Ziyi ^{[1
,2
,4
]}

Cheng, Jianfeng ^{[1
,2
,4
]}

Hu, Yongxin ^{[1
,2
,4
]}

Chen, Siyi ^{[1
,2
,4
]}

Qiu, Yu ^{[1
,2
,4
]}

Yang, Anshu ^{[1
,3
,4
]}

Wu, Zhihua ^{[1
,3
,4
]}

Li, Xin ^{[1
,2
,4
,5
]}

Chen, Hongbing ^{[1
,3
,4
]}

机构：

[1] Nanchang Univ, State Key Lab Food Sci & Resources, Nanchang 330047, Jiangxi, Peoples R China

[2] Nanchang Univ, Sch Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China

[3] Nanchang Univ, Sino German Joint Res Inst, Nanchang 330047, Jiangxi, Peoples R China

[4] Nanchang Univ, Jiangxi Prov Key Lab Food Allergy, Nanchang 330047, Jiangxi, Peoples R China

[5] Nanchang Univ, State Key Lab Food Sci & Resources, Nanchang 330047, Peoples R China

来源：

FOOD CHEMISTRY | 2024年 / 459卷

基金：

中国国家自然科学基金;

关键词：

Casein; Allergen; Hydrolysis; Allergenicity; Peptidomic analysis; IGG-BINDING EPITOPES; IDENTIFICATION; PROTEINS; PH; ALPHA(S1)-CASEIN; TEMPERATURE; RESPONSES;

D O I：

10.1016/j.foodchem.2024.140315

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Casein, the major allergen in cow's milk, presents a significant challenge in providing nutritional support for children with allergies. To address this issue, we investigated a composite enzyme, comprising papain and chymotrypsin, to reduce the allergenicity of casein. Enzymatic hydrolysis induced substantial structural changes in casein, diminishing its affinity for specific IgE and IgG antibodies. Additionally, in a BALB/c mouse model, casein hydrolysate alleviated allergic symptoms, evidenced by lower serum IgE and IgG levels, reduced plasma histamine, and decreased Th2 cytokine release during cell co-culture. Peptidomic analysis revealed a 52.38% and 60% reduction in peptides containing IgE epitopes in casein hydrolyzed by the composite enzyme compared to papain and chymotrypsin, respectively, along with a notable absence of previously reported T cell epitopes. These results demonstrate the potential of enzyme combinations to enhance the efficiency of epitope destruction in allergenic proteins, providing valuable insights into the development of hypoallergenic dairy products.

引用

页数：12