Lipid Droplet-Specific Dual-Response Fluorescent Probe for the Detection of Polarity and H2O2and Its Application in Living Cells

被引：0
作者：
Li M. [1 ]
Wang B. [1 ,2 ,3 ,4 ]
Liu J. [1 ]
Zhang Z. [1 ]
Chen L. [1 ,2 ,3 ,4 ]
Li Y. [1 ,3 ,4 ]
Yan X. [1 ,2 ,3 ,4 ]
机构：
[1] School of Chemical Engineering and Technology, Tianjin University, Tianjin
[2] Institute of Shaoxing, Tianjin University, Zhejiang, Shaoxing
[3] Guangdong Laboratory of Chemistry and Fine Chemical Industry Jieyang Center, Guangdong, Jieyang
[4] Tianjin Engineering Research Center of Functional Fine Chemicals, Tianjin
来源：
Analytical Chemistry | 2022年 / 94卷 / 27期
基金：
中国博士后科学基金; 中国国家自然科学基金;
关键词：
D O I：
10.1021/acs.analchem.2c01243
中图分类号：
学科分类号：
摘要：
H2O2and polarity are quite important in many physiological and pathological processes, and their relationship is complicated and obscure for researchers. Thus, it is vital and challenging to achieve simultaneous detection of H2O2and polarity in vivo. Herein, the first naphthalimide-triphenylamine-based dual-site fluorescent probe NATPA is developed for simultaneously imaging intracellular H2O2and polarity fluctuations. It exhibits excellent sensitivity (LOD = 44 nM), selectivity, and fast response (15 min) to H2O2and a superior capacity for detecting polarity upon the intramolecular charge transfer (ICT) effect. Besides, the probe displays low cytotoxicity and lipid droplet targeting and is further applied in imaging H2O2and polarity fluctuations in HepG2 and L-02 cells, so that NATPA is qualified to distinguish cancer cells from normal cells. This research contributes a new design principle for the construction of dual-site fluorescent probes for simultaneously detecting active molecules and polarity. © 2022 American Chemical Society. All rights reserved.
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页码：9732 / 9739
页数：7
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