Modulatory Effect of Polysaccharide from Artocarpus heterophyllus Lam. (Jackfruit) Pulp on Gut Microbiota in Mice

被引：0
|
作者：
Chen Y. [1 ,2 ]
Tan L. [2 ]
Liu Q. [3 ]
Wu G. [2 ,4 ]
Zhang Y. [2 ,4 ]
Xu F. [2 ,4 ]
Zhu K. [2 ,4 ]
机构：
[1] College of Food Science and Technology, Huazhong Agricultural University, Wuhan
[2] Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning
[3] Department of Pharmacology, School of Basic Medicine and Life Sciences, Hainan Medical College, Haikou
[4] Key Laboratory of Processing Suitability and Quality Control of the Special Tropical Crops of Hainan Province, Wanning
来源：
Science and Technology of Food Industry | 2022年 / 43卷 / 24期
关键词：
16S rRNA; gut microbiota; intestinal flora diversity; modulatory effect; polysaccharide from Artocarpus heterophyllus Lam; pulp (JFP-Ps);
D O I：
10.13386/j.issn1002-0306.2022030215
中图分类号：
学科分类号：
摘要：
：The present study aimed to investigate the modulatory effect of polysaccharide from Artocarpus heterophyllus Lam. pulp (JFP-Ps) on the diversity and structure of gut microbiota in mice. 24 male Kunming mice were randomly divided into four groups: Control group, JFP-Ps (50 mg/kg BW) group, JFP-Ps (100 mg/kg BW) group and JFP-Ps (200 mg/kg BW) group. The colonic feces of mice were collected after treating with JFP-Ps in different doses for 2 weeks. Then the DNA was extracted and the V3~V4 region of the 16S rRNA gene of the intestinal flora was amplified. The regulatory effect of JFP-Ps on the gut microbiota of mice was studied using Illumina Miseq high-throughput sequencing technology. The results showed that Bacteroidetes and Firmicutes were the dominant intestinal flora, and JFP-Ps modulated the composition of Bacteroidetes and Firmicutes in comparison with the control group. At the genus level, the abundance of SCFAs-producing bacteria, including Bacteroides, Allobaculum, Lactobacillus and Parabacteroides were significantly increased in response to JFP-Ps treatment, but a significant decrease in Prevotella. These results indicated that JFP-Ps could improve the diversity of gut microbiota by regulating the composition and structure of dominant bacteria such as Bacteroidetes and Firmicutes. This study would provide a theoretical basis for JFP-Ps in intestinal prebiotics. © 2022, Editorial Department of Science and Technology of Food Science. All rights reserved.
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页码：189 / 196
页数：7
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