Effect and Mechanism of Rosa roxburghii Fruit Polyphenols on Hypertension Induced by NG-Nitro-L-Arginine Methyl Ester in Mice

被引：0
作者：
Xu Q. [1 ]
Tan S. [1 ]
Yu L. [1 ]
Yuan M. [1 ]
Tan Y. [1 ]
Zhang L. [1 ]
机构：
[1] School of Liquor and Food Engineering, Guizhou University, Guizhou Rosa Roxburghii Research Institute, Guiyang
来源：
Shipin Kexue/Food Science | 2023年 / 44卷 / 17期
关键词：
endothelial function; hypertension; inflammation; oxidative stress; renin angiotensin aldosterone system; Rosa roxburghii fruit polyphenols;
D O I：
10.7506/spkx1002-6630-20220810-124
中图分类号：
学科分类号：
摘要：
Objective: To investigate the effect and possible mechanism of Rosa roxburghii fruit polyphenols (RRP) on hypertension induced by NG-nitro-L-arginine methyl ester (L-NAME) in mice. Methods: RRP with a purity of 68% was prepared in the laboratory. L-NAME was used to induce a mouse model of hypertension, and the mice were divided into four groups: model, positive control (captopril), low-dose RRP, and high-dose RRP. All mice were killed and dissected for measurement of organ indexes. Mice not treated with L-NAME served as a control group. The serum was collected to determine the levels of nitric oxide (NO), endothelin-1 (ET-1), renin, angiotensin II (Ang II), aldosterone (ALD), interleukin 6 (IL-6), and tumor necrosis factor-α (TNF-α). The levels of oxidative stress markers including malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were assayed in kidney and liver tissues, and heart and kidney tissues were stained with hematoxylin and eosin (HE) for pathological observation. Results: Compared with the blank group, the model group showed a significant increase in blood pressure; serum NO concentration significantly decreased (P < 0.05), serum IL-6 and TNF-α levels significantly increased (P < 0.05), and serum ET-1, renin, Ang II, and ALD levels significantly increased (P < 0.05). MDA levels in kidney and liver tissues significantly increased (P < 0.05), and SOD and GSH-Px activities significantly decreased (P < 0.05). Compared with the model group, blood pressure significantly increased in the positive control group and the low- and high-dose RRP groups (P < 0.05). Serum NO levels significantly increased (P < 0.05), and serum IL-6, TNF-α, ET-1, renin, Ang II, and ALD levels significantly decreased (P < 0.05). MDA levels in kidney and liver tissues significantly declined (P < 0.05), and GSH-Px activity significantly increased (P < 0.05). Conclusion: RRP has an obvious hypotensive effect, possibly by increasing NO bioavailability, improving endothelial cell dysfunction, inhibiting the over-activation of the renin angiotensin aldosterone system, and reducing inflammation and oxidative stress. © 2023 Chinese Chamber of Commerce. All rights reserved.
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页码：94 / 100
页数：6
相关论文
共 24 条
  • [1] LEWINGTON S, LACEY B, CLARKE R, Et al., The burden of hypertension and associated risk for cardiovascular mortality in China, JAMA Internal Medicine, 176, 4, pp. 524-532, (2016)
  • [2] DE MENEZES S T, GIATTI L, BRANT L C C, Et al., Hypertension, prehypertension, and hypertension control, Hypertension, 77, 2, pp. 672-681, (2021)
  • [3] 37, 5
  • [4] WANG H, CHEN Z, WANG M, Et al., The effect of polyphenol extract from Rosa roxburghii fruit on plasma metabolome and gut microbiota in type 2 diabetic mice, Foods, 11, 12, (2022)
  • [5] AZIS N A, AGARWAL R, ISMAIL N M, Et al., Blood pressure lowering effect of Ficus deltoidea var kunstleri in spontaneously hypertensive rats: possible involvement of renin-angiotensin-aldosterone system, endothelial function and anti-oxidant system, Molecular Biology Reports, 46, 3, pp. 2841-2849, (2019)
  • [6] HELMUTM H, NEALE J, BRIAN M, Et al., Polyphenol protection and treatment of hypertension, Phytomedicine, 23, 2, pp. 220-231, (2016)
  • [7] MORENO-LUNA R, MUNOZ-HERNANDEZ R, MIRANDA M L, Et al., Olive oil polyphenols decrease blood pressure and improve endothelial function in young women with mild hypertension, American Journal of Hypertension, 25, 12, pp. 1299-1304, (2012)
  • [8] YUAN Yao, LIU Taohua, YIN Lijuan, Et al., Polyphenol-rich extract from Litchi chinensis seeds alleviates hypertension-induced renal damage in rats, Journal of Agricultural and Food Chemistry, 69, 7, pp. 2138-2148, (2021)
  • [9] CHU H T, LI L, JIA M, Et al., Correlation between serum microRNA-136 levels and RAAS biochemical markers in patients with essential hypertension, European Review for Medical and Pharmacological Sciences, 24, 22, pp. 11761-11767, (2020)
  • [10] WILLIAMS B, MANCIA G, SPIERING W, Et al., 2018 ESC/ESH Guidelines for the management of arterial hypertension: the task force for the management of arterial hypertension of the European Society of Cardiology and the European Society of Hypertension, Journal of Hypertension, 36, 10, pp. 1953-2041, (2018)
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