Simultaneous immobilization enhances synergistic interactions and crude oil removal of bacterial consortium

被引：0

作者：

Rungsihiranrut A. ^{[1
,2
]}

Muangchinda C. ^{[1
,2
]}

Naloka K. ^{[2
,3
]}

Dechsakulwatana C. ^{[4
]}

Pinyakong O. ^{[2
,3
]}

机构：

[1] International Postgraduate Programs in Hazardous Substance and Environmental Management, Graduate School, Chulalongkorn University, Bangkok

[2] Center of Excellence in Microbial Technology for Marine Pollution Treatment (MiTMaPT), Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok

[3] Research Program on Remediation Technologies for Petroleum Contamination, Center of Excellence on Hazardous Substance Management (HSM), Bangkok

[4] Institute of Marine Science, Burapha University, Chonburi

来源：

Chemosphere | 2023年 / 340卷

关键词：

Bioremediation; Crude oil-contaminated seawater; Immobilized bacterial consortium; Oil spill; Whole genome analysis;

D O I：

10.1016/j.chemosphere.2023.139934

中图分类号：

学科分类号：

摘要：

Oil spillage has serious adverse effects on marine environments. The degradation of crude oil by microorganisms may be an effective and sustainable approach. In this study, the removal of crude oil from seawater by immobilized bacterial consortium was performed and the enhancement of crude oil degradation efficiency by varying immobilization methods and inoculum volume ratio was examined. The nonpathogenic and heavy metal-tolerant bacterial consortium of Sphingobium naphthae MO2-4 and Priestia aryabhattai TL01-2 was immobilized by biofilm formation on aquaporousgels. The simultaneous immobilization of strains MO2-4 and TL01-2 showed better crude oil removal efficiency than independent immobilization, which indicated positive interactions among consortium members in the mixed-culture immobilized systems. Moreover, the immobilized consortium at a 2:1 (MO2-4:TL01-2) inoculum volume ratio showed the best crude oil removal capacity. The immobilized consortium removed 77% of 2000 mg L−1 crude oil in seawater over 7 days. The immobilized consortium maintained crude oil removal efficacy in semicontinuous experiments. In addition, the immobilized consortium was used to remediate seawater contaminated with 1000 mg L−1 crude oil in a 20 L wave tank. After 28 days, the crude oil degradation efficiency of immobilized consortium was approximately 70%, and crude oil degradation through natural attenuation was not observed. Moreover, the genomic features of strains MO2-4 and TL01-2 are reported. Genomic analyses of both strains confirmed the presence of many genes involved in hydrocarbon degradation, heavy metal resistance, biosurfactant synthesis, and biofilm formation, supporting the biodegradation results and characterizing strain properties. The results of this work introduce the potential benefit of simultaneous immobilization of bacterial consortia to improve efficiency of crude oil biodegradation and has motivated further investigations into large-scale remediation of crude oil-contaminated seawater. © 2023 Elsevier Ltd

引用

共 49 条

[1]

Adofo Y.K., Nyankson E., Agyei-Tuffour B., Dispersants as an oil spill clean-up technique in the marine environment: a review, Heliyon, 8, 8, (2022)

[2]

Ahmed S.F., Mofijur M., Nuzhat S., Chowdhury A.T., Rafa N., Uddin M.A., Inayat A., Mahlia T.M.I., Ong H.C., Chia W.Y., Show P.L., Recent developments in physical, biological, chemical, and hybrid treatment techniques for removing emerging contaminants from wastewater, J. Hazard Mater., 416, (2021)

[3]

Ausuri J., Vitale G.A., Coppola D., Palma Esposito F., Buonocore C., de Pascale D., Assessment of the degradation potential and genomic insights towards phenanthrene by Dietzia psychralcaliphila JI1D, Microorganisms, 9, 6, (2021)

[4]

Ayangbenro A.S., Babalola O.O., Genomic analysis of Bacillus cereus NWUAB01 and its heavy metal removal from polluted soil, Sci. Rep., 10, 1, (2020)

[5]

Baati H., Siala M., Azri C., Ammar E., Dunlap C., Trigui M., Genomic analysis of heavy metal-resistant Halobacterium salinarum isolated from Sfax solar saltern sediments, Extremophiles, 26, 2, (2022)

[6]

Bhuyan R., Namdeo A., Verma J.S., Geed S.R., Isolation of indole biodegrading bacterial species from petroleum contaminated site of northeast India: growth kinetics and crude oil biodegradation assessment, J. Water Process Eng., 52, (2023)

[7]

Brettin T., Davis J.J., Disz T., Edwards R.A., Gerdes S., Olsen G.J., Olson R., Overbeek R., Parrello B., Pusch G.D., Shukla M., Thomason J.A., Stevens R., Vonstein V., Wattam A.R., Xia F., RASTtk: a modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes, Sci. Rep., 5, 1, (2015)

[8]

Dasgupta D., Ghosh R., Sengupta T.K., Biofilm-mediated enhanced crude oil degradation by newly isolated pseudomonas species, ISRN Biotechnol., 2013, (2013)

[9]

Dechsakulwatana C., Rungsihiranrut A., Muangchinda C., Ningthoujam R., Klankeo P., Pinyakong O., Biodegradation of petroleum oil using a constructed nonpathogenic and heavy metal-tolerant bacterial consortium isolated from marine sponges, J. Environ. Chem. Eng., 10, 6, (2022)

[10]

Etkin D.S., Nedwed T.J., Effectiveness of mechanical recovery for large offshore oil spills, Mar. Pollut. Bull., 163, (2021)

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