Mitigating effect of pomegranate peel extract against the furan induced testicular injury by apoptosis, steroidogenic enzymes and oxidative stress

被引：4
作者：
Ali W. [1 ,2 ,3 ,4 ]
Khatyan U. [4 ]
Sun J. [1 ,2 ,3 ]
Alasmari A. [5 ]
Alshahrani M.Y. [6 ]
Qazi I.H. [4 ]
Wang T. [1 ,2 ,3 ]
Liu Z. [1 ,2 ,3 ]
Zou H. [1 ,2 ,3 ]
机构：
[1] College of Veterinary Medicine, Yangzhou University Yangzhou, Jiangsu
[2] Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Jiangsu, Yangzhou
[3] Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Jiangsu, Yangzhou
[4] Sindh Agriculture University, Tandojam
[5] Biology Department, faculty of Science, University of Tabuk, Tabuk
[6] Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University
来源：
Chemosphere | 2024年 / 358卷
基金：
中国国家自然科学基金;
关键词：
Apoptosis; Furan; Inflammation; Oxidative stress; Pomegranate peel extract; Testicular injury;
D O I：
10.1016/j.chemosphere.2024.142086
中图分类号：
学科分类号：
摘要：
Furan is generated in a wide array of heat-treated foods through thermal degradation, leading to severe impairments in the male reproductive system. The main objective of this study was to investigate the potential of pomegranate peel extract (PGPE) in mitigating testicular dysfunctions induced by furan. Male rats were categorized into four groups: control/untreated, PGPE, furan, and PGPE + furan group. The study results revealed that furan-treated rats exhibited significantly elevated aminotransferase and phosphatase activity, and also generated increased oxidative stress, and reduced antioxidative stress protein activity. Additionally, protein content levels (ALT, AST, ALP, and ACP) and activities of steroidogenic Leydig cell hydroxysteroid dehydrogenase (3β-HSD and 17β-HSD) enzymes were significantly decreased. Significant variations in testicular parameters, apoptotic genes (Bcl-2, P53, and Caspase3), inflammatory and anti-inflammatory cytokines (IL1β, IL10), male sex hormones follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone, and sperm quality were also observed. Furthermore, testicular histological abnormalities were confirmed by biochemical and molecular modifications. Notably, PGPE pre-treated furan-intoxicated animals exhibited significant improvements in most of the assessed parameters compared to furan-treated groups. In conclusion, PGPE presents essential preventive measures and a novel pharmacological potential therapy against furan-induced testicular injury. © 2024
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