CT/bioluminescence dual-modal imaging tracking of stem cells labeled with Au@PEI@PEG nanotracers and RfLuc in nintedanib-assisted pulmonary fibrosis therapy

被引：6

作者：

Li X. ^{[1
,2
]}

Yu C. ^{[2
]}

Bao H. ^{[1
,2
]}

Chen Z. ^{[2
]}

Liu X. ^{[2
]}

Huang J. ^{[1
,2
]}

Zhang Z. ^{[1
,2
]}

机构：

[1] School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei

[2] CAS Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou

来源：

Nanomedicine: Nanotechnology, Biology, and Medicine | 2022年 / 41卷

基金：

中国国家自然科学基金;

关键词：

Au@PEI@PEG nanotracer; CT/bioluminescence imaging; IPF treatment; Mesenchymal stem cells; Nintedanib;

D O I：

10.1016/j.nano.2022.102517

中图分类号：

学科分类号：

摘要：

Mesenchymal stem cells (MSCs) are promising in idiopathic pulmonary fibrosis (IPF) therapy. However, low survival rate and ambiguous behavior of MSCs after transplantation impede their clinical translation. To this end, we have developed a new strategy to improve the survival rate and monitor the behavior of the transplanted MSCs simultaneously. In our strategy, nintedanib, a tyrosine kinase inhibitor, is employed to protect the human MSCs (hMSCs) from excessive oxidative stress responses and inflammatory environment in the damaged lung. Moreover, by labeling of the transplanted hMSCs with a computed tomography (CT) nanotracer, Au nanoparticles functionalized with polyethylenimine (PEI) and polyethylene glycol (PEG) (Au@PEI@PEG), in combination with red-emitting firefly luciferase (RfLuc), in vivo CT/bioluminescence (BL) dual-modal imaging tracking of the location, distribution, and survival of the transplanted hMSCs in presence of nintedanib were achieved, which facilitates the profound understanding of the role the stem cells play in IPF therapy. © 2022 Elsevier Inc.

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