Analysis on structure and hypoglycemic activity of Lanzhou lily polysaccharides from gradient alcoholic precipitation

被引：0

作者：

Zhang, Xinchi ^{[1
]}

Hui, Heping ^{[2
]}

Guo, Dongfei ^{[1
]}

Wei, Saixue ^{[1
]}

Guo, Min ^{[1
,3
,4
]}

Li, Xiaodong ^{[1
,3
,4
]}

机构：

[1] College of Pharmacy, Gansu University of Chinese Medicine, Gansu, Lanzhou,730010, China

[2] School of Biomedicine and Food Engineering, Shangluo University, Shaanxi, Shangluo,726000, China

[3] Gansu Provincial Hospital of Traditional Chinese Medicine, Gansu, Lanzhou,730050, China

[4] Professional Technical Center for Secondary Exploitation and Clinical Evaluation of Longyao Large Varieties, Gansu Academy of Traditional Chinese Medicine, Gansu, Lanzhou,730050, China

来源：

Jingxi Huagong/Fine Chemicals | 2023年 / 40卷 / 10期

关键词：

Amylases; -; Extraction; Glucose; Glycosylation; Medicine; Modernization; Precipitation; (chemical); (meteorology);

D O I：

10.13550/j.jxhg.20220961

中图分类号：

学科分类号：

摘要：

Four polysaccharides, named BLP-30, BLP-50, BLP-70 and BLP-80, respectively, were obtained from hot water extraction and alcohol precipitation with different volume fractions of ethanol (30%, 50%, 70% and 80%). The properties of the four polysaccharides were then tested, with their inhibitory ability on α-amylase and α-glucosidase compared and the correlation between the basic composition and their hypoglycemic activity analyzed. The results showed that all the four polysaccharides contained little or no uronic acid and protein, and BLP-80 had the highest amount of total soluble sugar (30.96%). All the four polysaccharides were mixed polysaccharides, with BLP-30 and BLP-50 glucans, BLP-70 galactomannan glucans and BLP-80 mannoglucan glucans, consisting of mannose, glucose and a small amount of galactose, while the mass average relative molecular mass gradually decreased from 625 kDa for BLP-30-1 to 2.45 kDa for BLP-70-4. The molar fractions of mannose, glucose and galactose in BLP-30 were 10.18%, 88.67% and 1.15%, respectively, in BLP-50 were 2.49%, 97.02% and 0.49%, respectively, and in BLP-70 were 26.94%, 68.21% and 4.85%, respectively. The mole fractions of mannose and glucose in BLP-80 were 41.56% and 58.44%, respectively. The inhibitory effects of the four polysaccharides on α-amylase and α-glucosidase gradually decreased in the following order: BLP-70>BLP-80>BLP-30>BLP-50 and BLP-50>BLP-70>BLP-80> BLP-30, respectively. The inhibitory ability of the four polysaccharides on α-amylase was positively correlated with the molar fraction of mannose and galactose, that of the polysaccharides on α-amylase was negatively correlated with the molar fraction of glucose, that of the polysaccharides on α-glucosidase was negatively correlated with the mass fraction of total soluble sugars and uronic acid, and the molar fraction of mannose, and that of polysaccharides on α-glucosidase was positively correlated with the molar fraction of glucose. © 2023 Fine Chemicals. All rights reserved.

引用

页码：2231 / 2240