Morphological changes in spores during germination in bacillus cereus and bacillus subtilis

被引：0
作者：
Tsugukuni T. [1 ,2 ]
Shigemune N. [2 ]
Nakayama M. [2 ]
Miyamoto T. [1 ]
机构：
[1] Division of Food Science and Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka
[2] Safety Science Research, Kao Corporation, 2606 Akabane, Ichikai-machi, Haga-gun, Tochigi
来源：
Biocontrol Science | 2021年 / 25卷 / 04期
关键词：
Germination; Morphological change; Spore;
D O I：
10.4265/BIO.25.203
中图分类号：
学科分类号：
摘要：
Processes from spore germination to outgrowth were observed in detail using scanning electron microscopy（SEM）and transmission electron microscopy（TEM）for Bacillus cereus and Bacillus subtilis. At 15 and 30 min after germination induction, SEM observation and SEM-EDX analysis of Bacillus spores prepared by freeze substitution showed that spherical structures including compounds having the same elemental ratio as that of the spore were observed on the surface of the spores. The results suggested the leakages of the cellular materials from the spores. At 360 min, B. cereus spores in outgrowth phase elongated with hemispherical structures at the end of the long side of the cells. The discoid structures with a hole（20-30 nm diameter）in the center was observed at 360 min. Confocal laser scanning microscopy after staining with fluorescence-labeled anti-spore antibodies showed that the hemispherical and discoid structures originated from the spore coat. These structures broke down after detached from the cells in outgrowth phase. © 2020 Society for Antibacterial and Antifungal Agents Japan. All rights reserved.
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页码：203 / 213
页数：10
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