Improved SDA production in high lipid accumulating strain of mucor circinelloides WJ11 by genetic modification

被引：0
|
作者：
Yang J. [1 ,2 ]
Khan M.A.K. [1 ]
López-García S. [2 ]
Nosheen S. [1 ]
Nazir Y. [1 ]
Zhang H. [1 ]
Garre V. [2 ]
Song Y. [1 ]
机构：
[1] Department of Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, Shandong
[2] Department of de Genética y Microbiología (Unidad Asociada al Instituto de Química Física Rocasolano, Consejo Superior de Investigaciones Científicas), Facultad de Biología, Universidad de Murcia, Murcia
来源：
Song, Yuanda (ysong@sdut.edu.cn) | 1600年 / Science Publications, 244, 5th Avenue # S-207,, New York, NY 10001, United States卷 / 16期
基金：
中国国家自然科学基金;
关键词：
Fatty Acid Desaturase 3; Homologous Overexpression; Mucor circinelloides; SDA Production;
D O I：
10.3844/ajbbsp.2020.138.147
中图分类号：
学科分类号：
摘要：
Stearidonic Acid (SDA; 18:4, n-3) is a ω-3 polyunsaturated fatty acid which is nutritionally important and has pharmaceutical applications. Hence, scientists are trying to construct SDA producing oleaginous microorganisms by genetic modification. Two enzymes, Delta-6 Desaturase (D6D) and ω-3 desaturase catalyze the reactions to produce SDA from Linoleic Acid (LA; 18:2, n-6). But the key enzyme, ω-3 desaturase converts LA to ALA and GLA to SDA is absent in high lipid accumulating Mucor circinelloides WJ11, retarding its ability to produce SDA. Therefore, in this study, we overexpressed fad3 gene from Arabidopsis thaliana in M. circinelloides WJ11 to produce SDA. Overexpression of fad3 gene in M. circinelloides WJ11 resulted in the production of 340 mg/l SDA. This research opened a new opportunity to make use of this fungus for industrial production of SDA. © 2020 Junhuan Yang, Md. Ahsanul Kabir Khan, Sergio López-García, Shaista Nosheen, Yusuf Nazir, Huaiyuan Zhang, Victoriano Garre and Yuanda Song.
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