A Colorimetric Biosensor Based on Hemin/G-quadruplex DNAzymes for the Detection of Sudan Dyes in Foods

被引：0

作者：

Lu C. ^{[1
]}

Yan S. ^{[2
]}

Liu C. ^{[3
]}

Lin X. ^{[4
]}

Wang S. ^{[1
]}

Feng X. ^{[1
]}

机构：

[1] School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing

[2] Institute of Agricultural Mechanization, Xinjiang Academy of Agricultural Sciences, Ürümqi

[3] Agricultural Products Processing Research Institute, Academy of Agriculture and Reclamation Science, Shihezi

[4] Xinjiang Shihezi Vocational Technical College, Shihezi

来源：

Shipin Kexue/Food Science | 2022年 / 43卷 / 22期

关键词：

Aptamer; Colorimetric biosensor; Hemin/G-quadruplex DNAzyme; Hybridization chain reaction; Sudan dyes;

D O I：

10.7506/spkx1002-6630-20220111-109

中图分类号：

学科分类号：

摘要：

A simple, novel, and sensitive biosensor for the colorimetric detection of Sudan dyes was successfully prepared using Sudan III aptamer as a recognition element, hemin/G-quadruplex DNAzyme as a signal indicator, and hybridization chain reaction as a signal amplification strategy. Under optimized conditions, the sensitivity, accuracy and specificity of the method were evaluated. Finally, the developed method was applied to detect Sudan III in food samples, and was validated by comparing it with the national standard method (GB/T 19681-2005). A good linear relationship was obtained between the absorbance at 450 nm and the concentration of Sudan III in the range of 0.5 to 250 ng/mL with correlation coefficient of 0.995, and the detection limit was 0.09 ng/mL. Specificity analysis demonstrated that this method could be used to detect Sudan IIV. The recoveries of Sudan III from spiked real samples ranged from 84.3% to 101.6%, with relative standard deviations of 4.13%-8.36%. There was no significant difference in the spiked recoveries between the proposed method and the national standard method (P > 0.05). This method was simple, reliable, sensitive, and suitable for the detection of Sudan dyes in food samples. © 2022, China Food Publishing Company. All right reserved.

引用

页码：346 / 352

页数：6

共 21 条

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