Screening of Trichoderma reesei Mutants and Optimization of Fermentation Conditions for Higher Cellulase Production

被引：0

作者：

Zou, Zongsheng ^{[1
]}

Wang, Jingya ^{[1
]}

Zhao, Yunying ^{[1
]}

Mao, Yin ^{[1
]}

Deng, Yu ^{[1
]}

机构：

[1] National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi,214122, China

来源：

Shipin Kexue/Food Science | 2019年 / 40卷 / 06期

关键词：

Genes;

D O I：

10.7506/spkx1002-6630-20180403-032

中图分类号：

R318.08 [生物材料学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 0805 ; 080501 ; 080502 ; 09 ;

摘要：

Mutants of Trichoderma reesei RUT-C30 induced by atmospheric and room temperature plasma (ARTP) were screened for cellulase production. Whole-genome sequencing was performed to analyze the mutation types of the selected mutant. Also, we investigated the optimization of fermentation medium and conditions for improved production of cellulase. Mutant JNDY-13 was found to produce the highest cellulase activity. Through shake-f l ask fermentation, the filter paper activity(FPA) of JNDY-13 was 2.21 IU/mL, which was 2.21 times higher than that of T. reesei RUT-C30. The highest FPA of 5.40 IU/mL was achieved through fed-batch fermentation in a 5-L fermentor under optimized conditions. A total of 752 mutations were identified in JNDY-13, and the 18-base pair insertion in the galactokinase gene may account for the increased cellulase activity. © 2019, China Food Publishing Company. All right reserved.

引用

页码：48 / 54

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