Electrochemical Aptasensor Based on Platinum @ Gold Nanowires as Signal Amplifier for Aflatoxin B1 Detection

被引：0
作者：
Liu J. [1 ]
Liu Z. [1 ]
Li X. [1 ]
Ma Z. [1 ]
机构：
[1] Faculty of Science, Henan University of Animal Husbandry and Economy, Zhengzhou
来源：
Science and Technology of Food Industry | 2023年 / 44卷 / 24期
关键词：
aflatoxin B1; aptamer; electrochemical aptasensor; platinum @ gold nanowires;
D O I：
10.13386/j.issn1002-0306.2023020181
中图分类号：
学科分类号：
摘要：
Objective: In this study, to meet food quality and safety and rapid determination of aflatoxin B1 (AFB1) content in food, an electrochemical aptamer-based sensor with plantium@gold nanowires (Pt@AuNWs) as the signal amplifier was developed for sensitive and selective detection of AFB1 in peanut. Method: Firstly, Au nanoparticles (AuNPs) were deposited on the surface of glassy carbon electrode (AuNPs/GC) for enhancing the electrocatalytic property of the sensor and linking complementary strand (cDNA) through Au-S bond. Then, the signal probes was constructed via template synthesis of Pt@AuNWs and combination with aptamer (Pt@AuNPs-apt), which could bind to the electrode based on base complementary pairing principle. In the presence of AFB1, it would compete with cDNA to bind aptamer and result in the detachment of some signal probes from the sensor surface, reducing the electrical signals generated by catalyzing H2O2, thereby the indirectly quantitative detection of AFB1 was achieved. Result: The sensor showed the best analytical performance with 0.25 nmol/L cDNA, incubating apt and cDNA for 40 min, 10 mmol/L H2O2, 0.1 mol/L pH7.0 PBS. Under optimization, this sensor showed good lineariy in the range of 1~100 ng/mL,with the calculated detection limit about 0.41 ng/mL. The present sensor delivered good sensitivity, selectivity and stability. The method was applied to the peanut samples for the recovery experiment and the recovery rate was 85.1%~88.0%. Conclusion: The constructed electrochemical adapter sensor can be applied to the detection of AFB1 in peanuts, and has great potential to be used in rapid detection. © 2023 Editorial Department of Science and Technology of Food Science. All Rights Reserved.
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页码：294 / 300
页数：6
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共 32 条
  • [1] WANG C, ZHAO Q., A reagentless electrochemical sensor for aflatoxin B1 with sensitive signal-on responses using aptamer with methylene blue label at specific internal thymine[J], Biosensors and Bioelectronics, 167, (2020)
  • [2] XU Z, LONG L L, CHEN Y Q, Et al., A nanozyme-linked immunosorbent assay based on metal–organic frameworks (MOFs) for sensitive detection of aflatoxin B1[J], Food Chemistry, 338, (2021)
  • [3] QIAN J, REN C C, WANG C Q, Et al., Gold nanoparticles mediated designing of versatile aptasensor for colorimetric/electrochemical dual-channel detection of aflatoxin B1[J], Biosensors and Bioelectronics, 166, (2020)
  • [4] LI S Q, ZHONG X Y, XU Y N, Et al., Smartphone-based reading system integrated with phycocyanin-enhanced latex nanospheres immunoassay for on-site determination of aflatoxin B1 in food stuffs[J]. [J], Food Chemistry, 360, (2021)
  • [5] SUN C N, LIAO X F, JIA B Y, Et al., Development of a ZnCdS@ZnS quantum dots-based label-free electrochemiluminescence immunosensor for sensitive determination of aflatoxin B1 in lotus seed[J], Microchimica Acta, 187, 4, pp. 236-244, (2020)
  • [6] JIANG S, ZHANG L X, LI J Z, Et al., Pressure/colorimetric dual-readout immunochromatographic test strip for point-of-care testing of aflatoxin B1[J], Talanta, 227, pp. 122207-122213, (2021)
  • [7] BU T, BAI F E, SUN X Y, Et al., An innovative prussian blue nanocubes decomposition-assisted signal amplification strategy suitable for competitive lateral flow immunoassay to sensitively detect aflatoxin B1[J], Food Chemistry, 344, (2021)
  • [8] GELL R M, CARBONE I., HPLC quantitation of aflatoxin B1 from fungal mycelium culture[J], Journal of Microbiological Methods, 158, (2019)
  • [9] ALGAMMAL A M, ELSAYED M E, HASHEM H R, Et al., Molecular and HPLC-based approaches for detection of aflatoxin B1 and ochratoxin A released from toxigenic Aspergillus species in processed meat[J], BMC Microbiology, 21, 1, (2021)
  • [10] ZHANG B, YU L, LIU Z, Et al., Rapid determination of aflatoxin B1 by an automated immunomagnetic bead purification sample pretreatment method combined with high-performance liquid chromatography[J], Journal of Separation Science, 43, 17, pp. 3509-3519, (2020)
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