The obesogen bisphenol A promotes adipogenesis in canine adipose-derived stem cells: Potential implication in dog obesity

被引：0
作者：
Park E.-J. [1 ,2 ]
Lee S. [3 ]
Kim J.-Y. [4 ]
Choi J. [1 ,2 ]
Lee Y.-S. [1 ,2 ]
Park M. [1 ,2 ]
Jeon J.-H. [3 ]
Lee H.-J. [1 ,2 ,4 ,5 ,6 ]
机构：
[1] Department of Food and Nutrition, College of BioNano Technology, Gachon University, Gyeonggi-do
[2] Institute for Aging and Clinical Nutrition Research, Gachon University, Gyeonggi-do
[3] Department of Physiology and Biomedical Sciences, Seoul National University College of Medicine, 103 Daehak-ro, Jongno-gu, Seoul
[4] Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Gyeonggi-do
[5] Department of Health Sciences and Technology, GAIHST, Gachon University, Incheon
[6] Gachon Biomedical Convergence Institute, Gachon University Gil Medical Center, Incheon
来源：
Chemosphere | 2024年 / 362卷
关键词：
Bisphenol A; cADSC; Dog toy; Obesogen; Transcriptome analysis;
D O I：
10.1016/j.chemosphere.2024.142579
中图分类号：
学科分类号：
摘要：
The growing number of companion dogs has contributed to a rapidly growing market for pet products, including dog toys. However, little is known about the hazardous substances released from dog toys. This study aims to examine the potential presence of obesogens, a subset of endocrine-disrupting chemicals (EDCs) that are widely utilized as raw materials in the manufacture of dog toy components, and their effects on dog health. To achieve this, we adapted and employed a migration method typically used for children's products to simulate obesogen exposure in dogs through sucking or chewing toys. We demonstrated that out of various obesogens, bisphenol A (BPA) was released from dog toys into synthetic saliva, whereas phthalates and azo dyes were not detected in any of the leachates. Additionally, we found that BPA induced adipogenic differentiation in canine adipose-derived stem cells (cADSCs). Our RNA sequencing experiments revealed that BPA alters the adipogenesis-related gene signature in cADSCs by elevating the expression levels of ADIPOQ, PLIN1, PCK1, CIDEC, and FABP4. The associated transcriptional changes are involved in the peroxisome proliferator-activated receptor (PPAR) signaling pathway, which may contribute to the promotion of adipogenesis by BPA. Our findings suggest that companion dogs are at risk of BPA exposure, which may contribute to obesity in dogs. Therefore, the implementation of precautionary measures is crucial. © 2024
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