Isolation and Identification of Latent Fungal Pathogens from Chinese Olive Fruit

被引：3
|
作者：
Chen P. [1 ,2 ,3 ]
Chen N. [1 ]
Lin H. [1 ,2 ,3 ]
Lin Y. [1 ,2 ,3 ]
Chen Y. [1 ,2 ,3 ]
机构：
[1] College of Food Science, Fujian Agriculture and Forestry University, Fuzhou
[2] Key Laboratory of Postharvest Biology of Subtropical Special Agricultural Products, Fujian Agriculture and Forestry University, Fujian Province University, Fuzhou
[3] Institute of Postharvest Technology of Agricultural Products, Fujian Agriculture and Forestry University, Fuzhou
来源：
Lin, Hetong (hetonglin@163.com) | 1600年 / Chinese Chamber of Commerce卷 / 41期
关键词：
Chinese olive; Fruit; Latent infection; Neofusicoccum parvum; Pestalotiopsis microspora; Postharvest disease;
D O I：
10.7506/spkx1002-6630-20191224-276
中图分类号：
学科分类号：
摘要：
In order to clarify the species of latent pathogenic fungi in Chinese olive fruit, the pathogenic fungi from the flower organ and fruit of 'Changying' Chinese olive (Canarium album (Lour.)Raeusch cv.Changying) were isolated by using tissue isolation, and their pathogenicity was assayed using Koch's postulates.The genomic (DNA of the isolated pathogens was extracted via the cetyl trimethyl ammonium bromide (CTAB) method Polymerase chain reaction (PCR) was employed to amplify of the ribosomal DNA internal transcribed spacer (rDNA-ITS) region, and then the amplified product was sequenced and subjected to homology analysis by basic local alignment search tool (BLAST).A phylogenetic tree for each of the six isolates was established using the neighbor-joining (NJ) method with MEGA6.0 software.According to their morphological and phylogenetic characteristics, the six latent pathogenic fungi were identified as Neofusicoccum parvum,Pestalotiopsis microspora, Aureobasidium pullulans, Phomopsis, Phanerochaete, and Guignardia with N.parvum and P.microspora being the dominant ones, which could infect the plant at the blooming stage.The results of this work can provide a scientific basis for controlling postharvest disease of Chinese olive fruit. © 2020, China Food Publishing Company. All right reserved.
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页码：165 / 171
页数：6
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