Safety Evaluation of Dairy-derived Enterococcus faecalis Isolates Based on Comparative Genomic Analysis

被引：0

作者：

Tingting S. ^{[1
]}

Xinwei M. ^{[1
]}

Jingda L. ^{[1
]}

Weicheng L. ^{[1
]}

Zhi Z. ^{[1
]}

机构：

[1] Inner Mongolia Agricultural University, Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Dairy Biotechnolo

来源：

Journal of Chinese Institute of Food Science and Technology | 2024年 / 24卷 / 05期

关键词：

comparative genomics; dairy products; Enterococcus faecalis; safety evaluation;

D O I：

10.16429/j.1009-7848.2024.05.030

中图分类号：

学科分类号：

摘要：

Enterococcus faecalis (E. faecalis) is a homofermentative lactic acid bacterium widely used in food production. However, many E. faecalis strains are opportunistic pathogens, leading to significant controversy regarding their use in foods. This study focuses on dairy-derived isolates of E. faecalis to explore their safety. Using the Illumina HiSeq high-throughput sequencing platform, we performed whole-genome sequencing on 35 dairy-derived E. faecalis isolates and conducted comparative genomics analysis with 21 fecal isolates downloaded from the NCBI database. The results showed that the overall genome length of E. faecalis was (2.93±0.13) Mb, GC content was (37.43±0.13)%, the number of transfer RNA (tRNA) was 56±4, and the number of coding sequences (CDS) was 2 768±146. There was no significant difference in the average genome length of E. faecalis milk isolates from fecal isolates, but the average number of CDS was significantly higher than that of fecal isolates. The phylogenetic tree results showed that the strains of the same segregation were clustered in the same evolutionary branch, which was basically consistent with the average nucleotide identity (ANI) clustering results. Compared with fecal isolates, each strain contained 3 less drug resistance genes and 2.4 more mobile genetic elements. Therefore, even E. faecalis isolated from dairy products has a risk of pathogenic disease and needs to undergo strict safety evaluation before use. © 2024 Chinese Institute of Food Science and Technology. All rights reserved.

引用

页码：359 / 372

页数：13

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