Effects of Biochar Combined with Different Types of Nitrogen Fertilizers on Denitrification Bacteria Community in Vegetable Soil

被引：0
|
作者：
Liu X.-W. [1 ]
Liu X.-R. [1 ]
Gao S.-J. [1 ]
Li G.-C. [1 ]
机构：
[1] Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing
来源：
Huanjing Kexue/Environmental Science | 2024年 / 45卷 / 04期
关键词：
ammonium nitrogen; biochar; denitrifying bacteria community; high-throughput sequencing; nitrate nitrogen;
D O I：
10.13227/j.hjkx.202304117
中图分类号：
学科分类号：
摘要：
To clarify the effects of biochar and different types of nitrogen fertilizers（ammonium and nitrate nitrogen fertilizers）on soil denitrifying bacterial communities，the treatments of no fertilizer（CK），（NH4）2SO（4 N1），（NH4）2SO4 + biochar［30 t·（hm2·a）-1］（N1B），single application of KNO（3 N2），and KNO3 + biochar［30 t·（hm2·a）-1］（N2B）were applied to vegetable soils in Shunyi. Soil samples were collected in winter，spring，and summer，and the community composition and diversity of denitrifying bacteria of narG，nirK，nirS，and nosZ types were analyzed using high-throughput sequencing to investigate the main influencing factors and to clarify the effects of biochar and different types of nitrogen fertilizers on the community composition and diversity of soil denitrifying bacteria. The results showed that：① Biochar and both types of nitrogen fertilizers could increase soil mineral N content，and compared with that under（NH4）2SO4 alone，biochar and（NH4）2SO4 increased soil NH4+-N content by 14. 6% and NO3--N content by 9. 4%. Compared with that under KNO3 alone，biochar and KNO3 increased soil NH4+-N content by 16. 0%，and NO3--N content increased by 15. 9%. ② The dominant phylum of the denitrifying bacterial community was Amoeba in all treatments，and its composition and dominant bacteria were affected by the application of biochar with both types of nitrogen fertilizers. The top three genera were Acidiphilium，Sulfuricaulis，and Klebsiella，respectively. The top three genera were Rhodopseudomonas，Microvirga，and Methylobacterium. ③ The application of biochar with two N fertilizers significantly affected the community composition and diversity of soil denitrifying bacteria，and soil pH，NO3--N，NH4+-N，and soil water content（SWC）were the main environmental factors affecting the community composition of soil denitrifying bacteria. The above study can provide a theoretical basis for reducing nitrate N accumulation and denitrification losses in vegetable soils and also provide a scientific basis for optimizing agricultural fertilizer management measures. © 2024 Science Press. All rights reserved.
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页码：2394 / 2405
页数：11
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