Screening and Identification Lactic Acid Bacteria with Inhibitory Activity against Quorum Sensing and Biofilm Formation of Aeromonas hydrophila from Traditional Fermented Vegetables

被引：0

作者：

Lin Y. ^{[1
]}

Cui T. ^{[1
]}

Lü X. ^{[1
,2
]}

Bai F. ^{[1
]}

Li J. ^{[1
]}

Shen L. ^{[3
]}

机构：

[1] College of Food Science and Technology, Bohai University, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou

[2] College of Biology Science and Technology, Beijing Forest University, Beijing

[3] Dalian Donglin Food Co., Ltd, Dalian, 116101, Liaoning

来源：

Journal of Chinese Institute of Food Science and Technology | 2019年 / 19卷 / 08期

关键词：

Aeromonas hydrophila; Biofilm; Inhibit; Lactic acid bacteria; Quorum sensing;

D O I：

10.16429/j.1009-7848.2019.08.023

中图分类号：

学科分类号：

摘要：

Objective: To screen lactic acid bacteria (LAB) with inhibitory activity against quorum sensing and biofilm formation of Aeromonas hydrophila from traditional fermented vegetables. Methods: Screening and degradation N-acyl-homoserine lactones (AHLs) of LAB was measured using Oxford cup agar well diffusion method, the inhibitory rate of biofilm was determined using the 96-well microtiter plates. The biofilm formation of A. hydrophila was observed by light microscopy and scanning electron microscope(SEM). Strain AJS2-4 was identified by biochemical tests and 16S rRNA sequence analysis. Results: Strain AJS2-4 with inhibitory activity toward quorum sensing of A. hydrophila was isolated from acid beans in Jixi, Anhui Province. 4 mg/mL crude extraction from strain AJS2-4 degraded the AHLs of A. hydrophila and degradation rate reached 49.36%, inhibited the biofilm formation of A. hydrophila and inhibitory rate reached 32.25%. 8 mg/mL crude extraction from strain AJS2-4 completely degraded the AHLs of A. hydrophila. The crude extraction from strain AJS2-4 remained inhibition activity after treatment at 121℃ for 30 min. However, its activity completely lost after treatment with protease. Therefore, the inhibition ingredient of crude extraction from strain AJS2-4 was preliminarily determined as proteins with heat-stable. Light microscopy revealed that crude extraction from strain AJS2-4 inhibited biofilm formation of A. hydrophila. The SEM images also exhibited that crude extraction from strain AJS2-4 not only decreased the production of biofilm of A. hydrophila, but also loosed the structure of biofilm. Strain AJS2-4 was identified as Lactobacillus plantarum by physiology and biochemical test and 16S rRNA sequence analysis. Conclusion: Lb. plantarum AJS2-4 with inhibitory activity toward quorum sensing and biofilm formation of A. hydrophila was isolated from acid beans in Jixi, Anhui Province. © 2019, Editorial Office of Journal of CIFST. All right reserved.

引用

页码：199 / 207

页数：8

共 15 条

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