In order to taking full advantage of Apostichopus japonica and developing its active ingredient. The paper studies the optimal conditions for enzymolysis of Apostichopus japonica and discusses the effect of the Apostichopus joponicus hydrolysate upon the xanthine oxidase. Method: To the degree of hydrolysis indicators, research enzyme hydrolysis process parameters by single factor experiment and optimize enzymatic process using response surface methodology, such as temperature, reaction time and the amount of, such as the single factor experiment. Method: Manufacturing model of hyperuricemia by feeding Yeast extract and oteracil potassium. After 30 days' feeding, measuring the activity of XOD and levels of UA and BUN in serum. Using gene chip technology, compare gene expression levels between different groups. While using RT-qPCR technology, verify gene chip results. Result: Selection protamex, reaction temperature 55℃, hydrolysis time 136 minutes, with enzyme amount 1.97%, the highest degree of hydrolysis. Apostichopus joponicus by hyperuricemia can reduce the levels of UA and BUN in rate's serum, they can reduce the activity of XOD and the activity of xanthine oxidase, inhibit the synthesis of uric acid production. And compare with model group differences in expression, these groups all have significant changes in the level of gene expression, 36 genes were up-regulated, 143 genes were down-regulated. Conclusion: Apostichopus japonica is rich in nourishment. Apostichopus japonica can inhibit the activity of xanthine oxidase and have a good function on hyperuricemia improvement. Therefore, Apostichopus japonica can be used as a good material of gout prevention. © 2019, Editorial Office of Journal of CIFST. All right reserved.
