Rapid screening of acetylcholinesterase active contaminants in water: A solid phase microextraction-based ligand fishing approach

被引：1
作者：
Huang Z. [1 ,2 ]
He L. [2 ]
Li H. [2 ]
Zhao J. [2 ]
Chen T. [2 ]
Feng Z. [2 ]
Li Y. [2 ]
You J. [2 ]
机构：
[1] The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health, Guizhou Medical University, Guian New Area
[2] Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou
来源：
Chemosphere | 2024年 / 356卷
基金：
中国国家自然科学基金;
关键词：
Acetylcholinesterase active compounds; Effect-directed analysis; Ligand fishing; Pesticides; Solid phase microextraction;
D O I：
10.1016/j.chemosphere.2024.141976
中图分类号：
学科分类号：
摘要：
Effect-directed analysis (EDA) has been increasingly used for screening toxic contaminants in the environment, but conventional EDA procedures are often time-consuming and labor-extensive. This challenges the use of EDA for toxicant identification in the scenarios when quick answers are demanded. Herein, a solid phase microextraction ligand fishing (SPME-LF) strategy has been proposed as a rapid EDA approach for identifying acetylcholinesterase (AChE) active compounds in water. The feasibility of ligand fishing techniques for screening AChE active chemicals from environmental mixtures was first verified by a membrane separation method. Then, SPME fibers were prepared through self-assembly of boronic acid groups with AChE via co-bonding and applied for SPME-LF. As AChE coated SPME fibers selectively enriched AChE-active compounds from water, comparing sorbing compounds by the SPME fibers with and without AChE coating can quickly distinguish AChE toxicants in mixtures. Compared with conventional EDA, SPME-LF does not require repeating sample separations and bioassays, endowing SPME-LF with the merits of low-cost, labor-saving, and user-friendly. It is believed that cost-efficient and easy-to-use SPME-LF strategy can potentially be a rapid EDA method for screening receptor-specific toxicants in aquatic environment, especially applicable in time-sensitive screening. © 2024 Elsevier Ltd
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共 32 条
[1]  
Brack W., Ait-Aissa S., Burgess R.M., Busch W., Creusot N., Di Paolo C., Escher B.I., Mark Hewitt L., Hilscherova K., Hollender J., Hollert H., Jonker W., Kool J., Lamoree M., Muschket M., Neumann S., Rostkowski P., Ruttkies C., Schollee J., Schymanski E.L., Schulze T., Seiler T.B., Tindall A.J., De Aragao Umbuzeiro G., Vrana B., Krauss M., Effect-directed analysis supporting monitoring of aquatic environments--An in-depth overview, Sci. Total Environ., 544, pp. 1073-1118, (2016)
[2]  
Chen G., Huang S., Kou X., Zhang J., Wang F., Zhu F., Ouyang G., Novel magnetic microprobe with benzoboroxole-modified flexible multisite arm for high-efficiency cis-diol biomolecule detection, Anal. Chem., 90, pp. 3387-3394, (2018)
[3]  
Chen G., Kou X., Huang S., Huang S., Zhang R., Liu C., Shen J., Zhu F., Ouyang G., Allochroic-graphene oxide linked 3D oriented surface imprinting strategy for glycoproteins assays, Adv. Funct. Mater., 28, (2018)
[4]  
Chen G., Qiu J., Fang X., Xu J., Cai S., Chen Q., Liu Y., Zhu F., Ouyang G., Boronate Affinity-molecularly imprinted biocompatible probe: an alternative for specific glucose monitoring, Chem. Asian J., 11, pp. 2240-2245, (2016)
[5]  
Chen L., Wang X., Liu Y., Di X., Dual-target screening of bioactive components from traditional Chinese medicines by hollow fiber-based ligand fishing combined with liquid chromatography-mass spectrometry, J. Pharm. Biomed. Anal., 143, pp. 269-276, (2017)
[6]  
Cheng F., Li H., Brooks B.W., You J., Signposts for aquatic toxicity evaluation in China: text mining using event-driven taxonomy within and among regions, Environ. Sci. Technol., 55, pp. 8977-8986, (2021)
[7]  
Cheng G., Pi Z., Zheng Z., Liu S., Liu Z., Song F., Magnetic nanoparticles-based lactate dehydrogenase microreactor as a drug discovery tool for rapid screening inhibitors from natural products, Talanta, 209, (2020)
[8]  
Dvir H., Silman I., Harel M., Rosenberry T.L., Sussman J.L., Acetylcholinesterase: from 3D structure to function, Chem. Biol. Interact., 187, pp. 10-22, (2010)
[9]  
Escher B.I., Stapleton H.M., Schymanski E.L., Tracking complex mixtures of chemicals in our changing environment, Science, 367, pp. 388-392, (2020)
[10]  
Gupta R., Mukherjee I., Doss R., Malik J., Milatovic D., Organophosphates and carbamates, Reproductive and Developmental Toxicology, (2017)
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