Affordable paper-based SWNTs field-effect transistor biosensors for nucleic acid amplification-free and label-free detection of micro RNAs

被引：0
作者：
Shen Y. [1 ]
Mulchandani A. [1 ,2 ]
机构：
[1] Department of Chemical and Environmental Engineering, University of California Riverside, 92521, CA
[2] Center for Environmental Research and Technology (CE-CERT), University of California Riverside, Riverside, 92507, CA
来源：
Biosensors and Bioelectronics: X | 2023年 / 14卷
关键词：
Field-effect transistor; Inkjet printing; Micro RNAs; NAA-free detection; Paper-based biosensor; SWNTs;
D O I：
10.1016/j.biosx.2023.100364
中图分类号：
学科分类号：
摘要：
Micro RNAs (miRNAs) are 19–23 nucleotides-long non-coding RNA which have been identified as important biomarkers for many diseases, including cancers. Here, we report an ultrasensitive, highly specific, label-free, and without nucleic acid amplification (NAA) detection of miRNAs using paper-based single-walled carbon nanotubes (SWNTs) field-effect transistor (FET) biosensors. The strategy involved a two-step protocol starting with direct hybridization of the target miRNA with a specific RNA probe immobilized on SWNTs networks deposited on the paper substrate to generate the first response, followed by the recognition of the resulting RNA/miRNA duplexes with the Carnation Italian ringspot virus p19 protein (p19) in a size-dependent manner, giving the second electrical response that magnified the biosensor sensitivity. As a demonstration, we detected the miRNA-122a, a promising biomarker for the diagnosis of early-stage hepatocellular carcinoma (HCC) in various sample matrices, including phosphate buffers, human serum, and synthetic saliva. We achieved the lowest detection of 0.1 aM and a wide dynamic range of 0.1 aM–1 fM, that demonstrated its potential for rapid, facile, low-cost, and point-of-care detection of miRNAs for early cancer diagnosis. © 2023 The Authors
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共 67 条
[51]  
Tian M., Qiao M., Shen C., Meng F., Frank L.A., Krasitskaya V.V., Wang T., Zhang X., Song R., Li Y., Liu J., Xu S., Wang J., Highly-sensitive graphene field effect transistor biosensor using PNA and DNA probes for RNA detection, Appl. Surf. Sci., 527, (2020)
[52]  
Tian R., Li Y., Bai J., Hierarchical assembled nanomaterial paper based analytical devices for simultaneously electrochemical detection of microRNAs, Anal. Chim. Acta, 1058, pp. 89-96, (2019)
[53]  
Torrente-Rodriguez R.M., Campuzano S., Lopez-Hernandez E., Granados R., Sanchez-Puelles J.M., Pingarron J.M., Direct determination of miR‐21 in total RNA extracted from breast cancer samples using magnetosensing platforms and the p19 viral protein as detector bioreceptor, Electroanalysis, 26, pp. 2080-2087, (2014)
[54]  
Tribolet L., Kerr E., Cowled C., Bean A.G.D., Stewart C.R., Dearnley M., Farr R.J., MicroRNA biomarkers for infectious diseases: from basic research to biosensing, Front. Microbiol., 11, (2020)
[55]  
Turner A., Karube I., Wilson G.S., Biosensors: Fundamentals and Applications, (1987)
[56]  
Varallyay E., Burgyan J., Havelda Z., MicroRNA detection by northern blotting using locked nucleic acid probes, Nat. Protoc., 3, (2008)
[57]  
Vargason J.M., Szittya G., Burgyan J., Hall T.M.T., Size selective recognition of siRNA by an RNA silencing suppressor, Cell, 115, pp. 799-811, (2003)
[58]  
Vu C.-A., Lai H.-Y., Chang C.-Y., Chan H.W.-H., Chen W.-Y., Optimizing surface modification of silicon nanowire field-effect transistors by polyethylene glycol for MicroRNA detection, Colloids Surf. B Biointerfaces, 209, (2022)
[59]  
Wallace R.B., Shaffer J., Murphy R.F., Bonner J., Hirose T., Itakura K., Hybridization of synthetic oligodeoxyribonucleotides to Φ X 174 DNA: the effect of single base pair mismatch, Nucleic Acids Res., 6, pp. 3543-3558, (1979)
[60]  
Wang J., Chen J., Sen S., MicroRNA as biomarkers and diagnostics, J. Cell. Physiol., 231, pp. 25-30, (2016)
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