Affordable paper-based SWNTs field-effect transistor biosensors for nucleic acid amplification-free and label-free detection of micro RNAs

被引：0

作者：

Shen Y. ^{[1
]}

Mulchandani A. ^{[1
,2
]}

机构：

[1] Department of Chemical and Environmental Engineering, University of California Riverside, 92521, CA

[2] Center for Environmental Research and Technology (CE-CERT), University of California Riverside, Riverside, 92507, CA

来源：

Biosensors and Bioelectronics: X | 2023年 / 14卷

关键词：

Field-effect transistor; Inkjet printing; Micro RNAs; NAA-free detection; Paper-based biosensor; SWNTs;

D O I：

10.1016/j.biosx.2023.100364

中图分类号：

学科分类号：

摘要：

Micro RNAs (miRNAs) are 19–23 nucleotides-long non-coding RNA which have been identified as important biomarkers for many diseases, including cancers. Here, we report an ultrasensitive, highly specific, label-free, and without nucleic acid amplification (NAA) detection of miRNAs using paper-based single-walled carbon nanotubes (SWNTs) field-effect transistor (FET) biosensors. The strategy involved a two-step protocol starting with direct hybridization of the target miRNA with a specific RNA probe immobilized on SWNTs networks deposited on the paper substrate to generate the first response, followed by the recognition of the resulting RNA/miRNA duplexes with the Carnation Italian ringspot virus p19 protein (p19) in a size-dependent manner, giving the second electrical response that magnified the biosensor sensitivity. As a demonstration, we detected the miRNA-122a, a promising biomarker for the diagnosis of early-stage hepatocellular carcinoma (HCC) in various sample matrices, including phosphate buffers, human serum, and synthetic saliva. We achieved the lowest detection of 0.1 aM and a wide dynamic range of 0.1 aM–1 fM, that demonstrated its potential for rapid, facile, low-cost, and point-of-care detection of miRNAs for early cancer diagnosis. © 2023 The Authors

引用

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