Expression and Molecular Evolution of Genes Related to Anthocyanin Biosynthesis in Blueberries

被引：3
|
作者：
Zheng J. [1 ,2 ]
Wang L. [2 ]
Li D. [2 ]
Chai Z. [2 ]
Zhang X. [1 ,2 ]
Huang W. [1 ,2 ]
机构：
[1] School of Food and Bioengineering, Jiangsu University, Zhenjiang
[2] Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing
来源：
Shipin Kexue/Food Science | 2022年 / 43卷 / 02期
关键词：
Blueberry anthocyanins; Gene expression; Structural genes; Transcription factor MYB;
D O I：
10.7506/spkx1002-6630-20201126-265
中图分类号：
学科分类号：
摘要：
In this study, we analyzed the relative expression levels of the structural genes VcCHS, VcCHI, VcF3H, VcDFR and VcANS as well as the transcription factor MYB involved in the anthocyanin biosynthesis pathways in six cultivars of highbush blueberry including Emerald, Lanmei #1, Gulf Coast, Legacy, Biloxi, and O'neal and two cultivars of rabbiteye blueberry (i.e. Brightwell and Gardenblue) by real-time polymerase chain reaction (real-time PCR). The results showed that VcCHS, VcCHI, VcF3H, VcDFR and VcANS could up-regulate the synthesis of anthocyanins and promote the production of anthocyanins. However, VcANR had a down-regulatory effect on anthocyanin synthesis, reducing the content of anthocyanins in blueberries. The regulatory effect of the transcription factor MYB on anthocyanin synthesis was relatively complex, which was related to its expression level. The results of this study can provide a foundation for further research on high-quality blueberry cultivars with respect to anthocyanins and for the development of functional foods containing blueberry anthocyanins. © 2022, China Food Publishing Company. All right reserved.
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页码：184 / 191
页数：7
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