Heparinized PGA host-guest hydrogel loaded with paracrine products from electrically stimulated adipose-derived mesenchymal stem cells for enhanced wound repair

被引：0
作者：
Li M. [1 ,3 ]
Li J. [2 ,3 ]
Xiong X. [1 ,3 ]
Wang Y. [2 ,3 ]
Jia Y.-G. [1 ,3 ,4 ]
Shi X. [1 ,3 ,4 ]
Fu X. [2 ,3 ,4 ]
机构：
[1] School of Materials Science and Engineering, South China University of Technology, Guangzhou
[2] School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou International Campus, Guangzhou
[3] National Engineering Research Center for Tissue Restoration and Reconstruction and Innovation Center for Tissue Restoration and Reconstruction, Guangzhou
[4] Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou
来源：
Engineered Regeneration | 2023年 / 4卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Electrical stimulation; Heparinized PGA host-guest hydrogel; Paracrine factors; Wound repair;
D O I：
10.1016/j.engreg.2023.03.002
中图分类号：
学科分类号：
摘要：
The microenvironment of the wound bed is essential in the regulation of wound repair. In this regard, strategies that provide a repairing favorable microenvironment may effectively improve healing outcomes. Herein, we attempted to use electrical stimulation (ES) to boost the paracrine function of adipose-derived stem cells from rats (rASCs). By examining the concentrations of two important growth factors, VEGF and PDGF-AA, in the cell culture supernatant, we found that ES, especially 5 μA ES, stimulated rASCs to produce more paracrine factors (5 μA-PFs). Further studies showed that ES may modulate the paracrine properties of rASCs by upregulating the levels of TRPV2 and TRPV3, thereby inducing intracellular Ca2+ influx. To deliver the PFs to the wound to effectively improve the wound microenvironment, we prepared a heparinized PGA host-guest hydrogel (PGA-Hp hydrogel). Moreover, PGA-Hp hydrogel loaded with 5 μA-PFs effectively accelerated the repair process of the full-thickness wound model in rats. Our findings revealed the effects of ES on the paracrine properties of rASCs and highlighted the potential application of heparinized PGA host-guest hydrogels loaded with PFs derived from electrically stimulated rASCs in wound repair. © 2023
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页码：225 / 237
页数：12
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