A Colorimetric Aptamer Sensor Based on Magnetic Nanoparticle for the Detection of Ochratoxin A

被引：0

作者：

Qi X. ^{[1
]}

Wang J. ^{[2
]}

Zou T. ^{[2
]}

Zhu L. ^{[2
]}

Zhang X. ^{[2
]}

Kan J. ^{[1
]}

Li Q. ^{[2
]}

机构：

[1] College of Food Science and Technology, Jiangsu Agri-animal Husbandry Vocational College, Taizhou

[2] School of Food Science and Engineering, Yangzhou University, Yangzhou

来源：

Science and Technology of Food Industry | 2024年 / 45卷 / 11期

关键词：

aptamer; colorimetric sensor; magnetic nanoparticle; ochratoxin A; ratio;

D O I：

10.13386/j.issn1002-0306.2023080021

中图分类号：

学科分类号：

摘要：

A novel ratio colorimetric sensor based on magnetic nanoparticles (Fe3O4@Au) and silver nanoparticles (Ag NPs) was constructed for the detection of Ochratoxin A (OTA) by complementary DNA base pairing. The OTA aptamer coupled Fe3O4@Au was served as reference signal, and complementary DNA (cDNA) coupled Ag NPs was served as the response signal. A ratio nanoprobe (Fe3O4@Au-Ag) with two UV absorption peaks was prepared by regulating the ratio and DNA base complementarity. In absence of OTA, the absorbance peak ratio (A400/A580) of the ratio colorimetric sensor could maintain a constant value. The detection conditions (time, pH, and temperature) were optimized, and under optimized conditions, the sensor showed a good linear relationship to OTA detection, with a linear range of 0.01 ng·mL−1 to 1000 ng· mL−1 and a detection limit of 0.033 ng·mL−1. The sensor had good selectivity, reproducibility and high sensitivity, which was successfully applied to the detection of OTA in pea powder with a recovery rate of 93.9%~96.0%. © The Author(s) 2024.

引用

页码：253 / 259

页数：6

共 30 条

[1]

WANG H, ZHAO B B, YE Y F, Et al., A fluorescence and surface-enhanced Raman scattering dual-mode aptasensor for rapid and sensitive detection of ochratoxin A[J], Biosensors and Bioelectronics, 207, (2022)

[2]

KHOSHBIN Z, ABNOUS K, TAGHDISI S M, Et al., A novel liquid crystal-based aptasensor for ultra-low detection of ochratoxin a using a π-shaped DNA structure:Promising for future on-site detection test strips[J], Biosensors and Bioelectronics, 191, (2021)

[3]

WANG Y H, SONG W, ZHAO H Y, Et al., DNA walker-assisted aptasensor for highly sensitive determination of ochratoxin A[J], Biosensors and Bioelectronics, 182, (2021)

[4]

BAO K L, LIU X, XU Q, Et al., Nanobody multimerization strategy to enhance the sensitivity of competitive ELISA for detection of ochratoxin A in coffee samples[J], Food Control, 127, (2021)

[5]

HAO L L, WANG W, SHEN X Q, Et al., A fluorescent DNA hydrogel aptasensor based on the self-assembly of rolling circle amplification products for sensitive detection of ochratoxin A[J], Journal of Agricultural and Food Chemistry, 68, 1, (2020)

[6]

MOEZ E, NOEL D, BRICE S, Et al., Aptamer assisted ultrafiltration cleanup with high performance liquid chromatography-fluorescence detector for the determination of OTA in green coffee[J], Food Chemistry, 310, (2020)

[7]

NAO S C, WU K J, WANG W H, Et al., Recent progress and development of g-quadruplex-based luminescent assays for ochratoxin A detection[J], Frontiers in Chemistry, 8, (2020)

[8]

ZHANG X X, LI M H, CHENG Z, Et al., A comparison of electronic nose and gas chromatography-mass spectrometry on discrimination and prediction of ochratoxin A content in Aspergillus carbonarius cultured grape-based medium[J], Food Chemistry, 297, (2019)

[9]

ALHAMOUD Y, YANG D T, KENSTON S S F, Et al., Advances in biosensors for the detection of ochratoxin A:Bio-receptors, nanomaterials, and their applications[J], Biosensors and Bioelectronics, 141, (2019)

[10]

WANG S, ZHANG Y J, PANG G S, Et al., Tuning the aggregation/disaggregation behavior of graphene quantum dots by structure-switching aptamer for high-sensitivity fluorescent ochratoxin A sensor[J], Analytical Chemistry, 89, 3, (2017)

← 1 2 3 →