Influence of Fermentation on the Alleviative Effect of Glucosinolates from Broccoli Florets on Oxidative Stress in Caenorhabditis elegans

被引:0
作者
Wang X. [1 ,2 ]
Liu Y. [1 ,2 ]
Yu J. [1 ]
Tan Z. [1 ,2 ]
Xia X. [1 ]
Zhao J. [1 ]
Ming J. [1 ]
机构
[1] College of Food Science, Southwest University, Chongqing
[2] National Demonstration Center for Experimental Food Science and Technology Education, Southwest University, Chongqing
来源
Shipin Kexue/Food Science | 2023年 / 44卷 / 05期
关键词
antioxidant capacity; broccoli glucosinolate; Caenorhabditis elegans; fermentation;
D O I
10.7506/spkx1002-6630-20220406-064
中图分类号
学科分类号
摘要
To investigate the effect of lactic acid bacteria (LAB) fermentation on the antioxidant activity of glucosinolates from broccoli florets, the glucosinolate composition and in vitro antioxidant activity of broccoli florets fermented by Lactobacillus plantarum were determined, and the effect of glucosinolates extracted from fermented broccoli florets on oxidative stress resistance and antioxidant indicators in Caenorhabditis elegans was evaluated. Results showed that LAB fermentation improved the in vitro antioxidant activity of broccoli glucosinolates. Glucosinolates from fermented broccoli could increase the survival rate of C. elegans under heat and oxidative stress, significantly reduce the levels of reactive oxygen species (ROS) and lipofuscin (P < 0.05), and increase the activity of superoxide dismutase (SOD), thereby alleviating oxidative damage in C. elegans. To sum up, LAB fermentation can help to improve the antioxidant activity of broccoli glucosinolate, which will provide a theoretical basis for the development of glucosinolates from cruciferous vegetables. © 2023 Chinese Chamber of Commerce. All rights reserved.
引用
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页码:205 / 213
页数:8
相关论文
共 39 条
  • [1] NEMOTO S, TAKEDA K, YU Z X, Et al., Role for mitochondrial oxidants as regulators of cellular metabolism, Molecular and Cellular Biology, 20, 19, pp. 7311-7318, (2000)
  • [2] LIGUORI I, RUSSO G, CURCIO F, Et al., Oxidative stress, aging, and diseases, Clinical Interventions in Aging, 13, pp. 757-772, (2018)
  • [3] RASHEED Z, ALHARBI A, ALRAKEBEH A, Et al., Thymoquinone provides structural protection of human hemoglobin against oxidative damage: biochemical studies, Biochimie, 192, pp. 102-110, (2022)
  • [4] LU Q, LIU W J, YANG L, Et al., Investigation of the effects of different organosolv pulping methods on antioxidant capacity and extraction efficiency of lignin, Food Chemistry, 131, 1, pp. 313-317, (2012)
  • [5] GU Y J, GUO Q H, ZHANG L, Et al., Physiological and biochemical metabolism of germinating broccoli seeds and sprouts, Journal of Agricultural and Food Chemistry, 60, 1, pp. 209-213, (2012)
  • [6] GROENBAEK M, TYBIRK E, KRISTENSEN H L., Glucosinolate and carotenoid content of white- and yellow-flowering rapeseed grown for human consumption as sprouts and seedlings under light emitting diodes, European Food Research and Technology, 244, 6, pp. 1121-1131, (2018)
  • [7] HUNZIKER P, HALKIER B A, SCHULZ A., Arabidopsis glucosinolate storage cells transform into phloem fibres at late stages of development, Journal of Experimental Botany, 70, 16, pp. 4305-4317, (2019)
  • [8] VANDUCHOVA A, ANZENBACHER P, ANZENBACHEROVA E., Isothiocyanate from broccoli, sulforaphane, and its properties, Journal of Medicinal Food, 22, 2, pp. 121-126, (2019)
  • [9] MASTUO T, MIYATA Y, YUNO T, Et al., Molecular mechanisms of the anti-cancer effects of isothiocyanates from cruciferous vegetables in bladder cancer, Molecules, 25, 3, (2020)
  • [10] SUBEDI L, LEE J H, YUMNAM S, Et al., Anti-inflammatory effect of sulforaphane on LPS-activated microglia potentially through JNK/AP-1/NF-kappaB inhibition and Nrf2/HO-1 activation, Cells, 8, 2, (2019)