Determination of Short-Chain Fatty Acids and Lactic Acid in Biosamples by High Performance Liquid Chromatography with Pre-Column Derivatization

被引：0

作者：

Wang G. ^{[1
]}

Huang W. ^{[1
]}

Huo J. ^{[1
]}

Yan M. ^{[1
]}

Wu S. ^{[1
]}

Hao Z. ^{[1
]}

Wei Y. ^{[1
]}

机构：

[1] Quantum Hi-Tech (Guangdong) Biological Co. Ltd., Jiangmen

来源：

Shipin Kexue/Food Science | 2022年 / 43卷 / 18期

关键词：

Cecal contents; Feces; Gas chromatography; High performance liquid chromatography; Lactic acid; Pre-column derivatization; Serum; Short-chain fatty acids;

D O I：

10.7506/spkx1002-6630-20210923-258

中图分类号：

学科分类号：

摘要：

A pre-column derivatization high performance liquid chromatography (PCD-HPLC) method was developed for simultaneous analysis of short-chain fatty acids (SCFAs) and lactic acid in complex biological samples including intestinal contents and serum. The contents of SCFAs and lactate in mouse feces were determined by PCD-HPLC and compared with those determined by gas chromatography (GC) and high performance liquid chromatography (HPLC). Then, methodological evaluation was performed on PCD-HPLC. PCD-HPLC was applied to analyze intestinal contents and blood. Results showed that GC could rapidly detect acetate, propionate, butyrate and valerate, but not lactic acid. HPLC yielded many impurity peaks and inaccurate results for lactic acid. The PCD-HPLC method was applicable to a wide range of samples and allowed for simultaneous analysis of lactate and five SCFAs in intestinal contents and serum. Only a few impurity peaks appeared in the chromatogram, and a good separation of all analytes was achieved. PCD-HPLC was accurate, giving a coefficient of variation (CV) less than or equal to 8.87% for each analyte. In conclusion, this method is superior to GC and HPLC in determining SCFAs and lactic acid in complicated biological samples such as intestinal contents and serum, and can meet the demand for accurate analysis of SCFAs and lactic acid in complicated biological samples in response to the rapid development of microbial metabolomics. © 2022, China Food Publishing Company. All right reserved.

引用

页码：265 / 271

页数：6

共 39 条

[1]

LEONE V, CHANG E B, DEVKOTA S., Diet, microbes, and host genetics: the perfect storm in inflammatory bowel diseases, Journal of Gastroenterology, 48, 3, pp. 315-321, (2013)

[2]

BORRE Y E, O'KEEFFE G W, CLARKE G, Et al., Microbiota and neurodevelopmental windows: implications for brain disorders, Trends in Molecular Medicine, 20, 9, pp. 509-518, (2014)

[3]

FUNG T C, OLSON C A, HSIAO E Y., Interactions between the microbiota, immune and nervous systems in health and disease, Nature Neuroscience, 20, pp. 145-155, (2017)

[4]

WU H, TREMAROLI V, BACKHED F., Linking microbiota to human diseases: a systems biology perspective, Trends in Endocrinology &Metabolism, 26, 12, pp. 758-770, (2015)

[5]

SEKIROV I, RUSSELL S L, ANTUNES L C M, Et al., Gut microbiota in health and disease, Physiological Reviews, 90, 3, pp. 859-904, (2010)

[6]

NISHITSUJI K, XIAO J Z, NAGATOMO R, Et al., Analysis of the gut microbiome and plasma short-chain fatty acid profiles in a spontaneous mouse model of metabolic syndrome[J], Scientific Reports, 7, 1, (2017)

[7]

HAMER H M, JONKERS D, VENEMA K, Et al., Review article: the role of butyrate on colonic function, Alimentary Pharmacology and Therapeutics, 27, 2, pp. 104-119, (2008)

[8]

KUMAR J, RANI K, DATT C., Molecular link between dietary fibre, gut microbiota and health[J], Molecular Biology Reports, 47, 8, pp. 6229-6237, (2020)

[9]

SIVAPRAKASAM S, PRASAD P D, SINGH N., Benefits of short-chain fatty acids and their receptors in inflammation and carcinogenesis, Pharmacology & Therapeutics, 164, pp. 144-151, (2016)

[10]

WOLEVER T M S, JOSSE R G, LEITER L A, Et al., Time of day and glucose tolerance status affect serum short-chain fatty acid concentrations in humans, Metabolism, 46, 7, pp. 805-811, (1997)

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