Dynamic characters of structure of designer cellulosome and enzymolysis of steam explosion corn stover

被引：0
|
作者：
Du J. [1 ]
Liu L. [1 ]
Wan P. [1 ]
Li Y. [1 ]
Li J. [1 ]
Tian S. [1 ]
机构：
[1] College of Life Science, Capital Normal University, Beijing
来源：
Taiyangneng Xuebao/Acta Energiae Solaris Sinica | 2021年 / 42卷 / 12期
关键词：
Cellulase; Cellulosic ethanol; Corn stover; Designer cellulosome; Kinetics; Thermal stability;
D O I：
10.19912/j.0254-0096.tynxb.2019-1390
中图分类号：
学科分类号：
摘要：
To investigate the effect of the structure of designer cellulosome on the enzymatic kinetics, the designer cellulosome with different structures were constructed through extracellular self-assembly by anchoring cellulases to the primary scaffoldin ScafI-CBM3a or the adaptor scaffoldin ScafII-CipA and ScafII-ScaB. The thermostability difference between designer cellulosome and free enzymes was analyzed comparatively, the enzymatic kinetic analysis of steam explosion corn stover was carried out, the Michaelis constant(Km), maximum reaction rate(Vmax) and substrate feedback inhibition constant(K)were obtained finally. As the results, the binding of cellulase and scaffold protein can reduce its thermal sensitivity and improve its thermal stability. The steam explosion corn stover of designer cellulosome with secondary scaffold protein has the value of Km=0.02 mg/mL, Vmax=19 mg/(mL•min), K=0.04 mg/L. It indicates that the affinity between enzymes and substrates enhanced by secondary scaffold proteins is higher than that of the simple structure, Km increases by 19.8%. The complex spatial structure of designer cellulosome can change the enzymatic kinetics characteristics, even improve the thermostability of the cellulase. © 2021, Solar Energy Periodical Office Co., Ltd. All right reserved.
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页码：292 / 296
页数：4
相关论文
共 14 条
  • [1] KAHN A, MORAIS S, GALANOPOULOU A P, Et al., Creation of a functional hyperthermostable designer cellulosome, Biotechnology for biofuels, 12, 44, pp. 1-15, (2019)
  • [2] GARCiA A B, MELERO R, DIAS F M, Et al., Molecular architecture and structural transitions of a Clostridium thermocellum mini-cellulosome, Journal of molecular biology, 407, 4, pp. 571-580, (2011)
  • [3] GALANOPOULOU A P, MORAIS S, GEORGOULIS A, Et al., Insights into the functionality and stability of designer cellulosomes at elevated temperatures, Applied microbiology and biotechnology, 100, 20, pp. 8731-8743, (2016)
  • [4] WILLSON B J, KOVaCS K, TOM W S, Et al., Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC824, Biotechnology for biofuels, 9, 109, pp. 1-22, (2016)
  • [5] TANG H T, HOU J, SHEN Y, Et al., Research Advances on cellulosome chimeras in Saccharomyces cerevisiae, Chinese journal of bioprocess engineering, 12, 1, pp. 94-101, (2014)
  • [6] WANG J L, WANG L S, LIU W F, Et al., Research advances on the assembly mode of cellulosomal macromolecular complexes, Progress in biochemistry and biophysics, 38, 1, pp. 28-35, (2011)
  • [7] TSAI S L, DASILVA N A, CHEN W., Functional display of complex cellulosomes on the yeast surface via adaptive assembly, ACS synthetic biology, 2, 1, pp. 14-21, (2013)
  • [8] TIAN S, DU J L, BAI Z S, Et al., Design and construction of synthetic cellulosome with three adaptor scaffoldins for cellulosic ethanol production from steam-exploded corn stover, Cellulose, 26, 15, pp. 8401-8415, (2019)
  • [9] WANG Z, LYU Z, DU J L, Et al., Combined process for ethanol fermentation at high-solids loading and biogas digestion from unwashed steam-exploded corn stover, Bioresource technology, 166, pp. 282-287, (2014)
  • [10] MO C L, CHEN N, LYU T, Et al., Direct ethanol production from steam-exploded corn stover using a synthetic diploid cellulase-displaying yeast consortium, Bioresources, 10, 3, pp. 4460-4472, (2015)
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