Distribution Characteristics of Antibiotic Resistance Genes in PM 2.5 of a Concentrated Broiler Feeding Operation

被引：2
作者：
Liu F. [1 ]
Xu X. [1 ]
Tu B.-W. [2 ]
Wang C.-Q. [1 ]
Jiang X.-D. [1 ]
Wang L.-P. [1 ]
Xue Y.-G. [1 ,3 ]
机构：
[1] School of Environmental and Safety Engineering, Changzhou University, Changzhou
[2] Changzhou Centers for Disease Control and Prevention, Changzhou
[3] Key Laboratory of Environmental Protection of Water Environment Biological Monitoring of Jiangsu Province, Changzhou Environmental Monitoring Center of Jiangsu Province, Changzhou
来源：
Huanjing Kexue/Environmental Science | 2019年 / 40卷 / 02期
关键词：
Antibiotic resistance genes; Broiler feeding operation; Class I integrin; Feces; PM [!sub]2.5[!/sub;
D O I：
10.13227/j.hjkx.201808017
中图分类号：
学科分类号：
摘要：
Concentrated poultry feeding operations are an important source of antibiotic resistance genes (ARGs). Little attention has been given to PM 2.5 as a mechanism for exposing ARGs to humans. In this study, PM 2.5 and fecal samples from inside the broiler feeding operation and PM 2.5 samples from outside the broiler feeding operation were collected. All samples were subjected to the determination of class Ⅰ integrin (intI1), total bacterial gene (16S rDNA), and 19 ARGs of six types by quantitative real-time PCR (qPCR). The results indicated that, excluding bla GES-1 and bla SHV-1 , the remaining 17 ARGs were detected in all six samples. Sulfonamide resistance genes, tetracycline resistance genes, macrolide resistance genes, and aminoglycoside resistance genes were abundant in the feces, reaching 1.04×10 9 -3.27×10 10 copies•g -1 , while feces was an important source of antibiotic resistance genes in PM 2.5 of the broiler feeding operation. There were high abundances of sulfonamide resistance genes and macrolide resistance genes in PM 2.5 from inside the broiler feeding operation, reaching (8.9±1.9)×10 7 copies•m -3 and (5.6±3.1)×10 7 copies•m -3 , respectively. The abundance of ARGs in the PM 2.5 samples from inside the broiler feeding operation was significantly higher compared to the outside PM 2.5 samples. There was a significant positive correlation between PM 2.5 mass concentration and 16S rDNA, intI1, and ARGs abundance, indicating that PM 2.5 was the reservoir and disseminator of airborne bacteria, ARGs, and intI1 in the broiler feeding operation. The abundance of intI1 was higher than ARGs among all samples, and the co-occurring relationship between intI1 and ARGs demonstrates the threat of multi-drug resistance, which is harmful to the surrounding air environment and the health of the breeder and poultry. © 2019, Science Press. All right reserved.
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页码：567 / 572
页数：5
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