Effects of Nitrogen and Phosphorus Additions on Soil Microorganisms in Saline-alkaline Grassland

被引：0

作者：

Yang J.-Q. ^{[1
]}

Diao H.-J. ^{[2
,3
,4
]}

Hu S.-Y. ^{[3
,5
]}

Chen X.-P. ^{[2
,3
,4
]}

Wang C.-H. ^{[2
,3
,4
]}

机构：

[1] College of Life Sciences, Shanxi Agricultural University, Taigu

[2] College of Grassland Science, Shanxi Agricultural University, Taigu

[3] State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing

[4] Youyu Loess Plateau Grassland Ecosystem Research Station, Youyu

[5] University of Chinese Academy of Sciences, Beijing

来源：

Huanjing Kexue/Environmental Science | 2021年 / 42卷 / 12期

关键词：

Ammonia-oxidizing microorganisms; Bacteria; Fungi; Nitrogen and phosphorus addition; Saline-alkaline grassland;

D O I：

10.13227/j.hjkx.202105003

中图分类号：

学科分类号：

摘要：

Soil microbial composition and community structure in salinization grassland are substantially different from those of non-saline-alkaline natural grassland due to the lower soil nutrients, inferior natural conditions, and higher soil salinization stress. Nitrogen (N) and phosphorus (P) are main factors that limit the productivity in semi-arid grassland, and whether soil microorganisms are affected by the addition of N and P in nutrient limitation and salinization grassland is still unclear. This experiment was conducted in Youyu Loess Plateau Grassland Ecosystem Research Station, Shanxi Province. Four treatments were set in 2017, which included the control (CK), N addition (N), P addition (P), and N and P addition (NP). The ammonia-oxidizing microorganisms [i.e., ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA)], soil bacteria (B) and fungi (F) composition, and soil microbial biomass carbon and nitrogen (MBC and MBN, respectively), as well as soil pH and base cations, were measured during the growing season in 2020. Our results showed that: ① the sampling month had the main effect on soil ammonia-oxidizing microorganisms, soil bacterial and fungal composition, and microbial biomass, which were regulated by precipitation distribution, soil water content, and plant factors. ② Compared with that under the control, the ratio of AOA/AOB was significantly decreased by 51% under N addition, whereas P addition had no significant effect on soil microbial characteristics (ammonia-oxidizing microorganisms, bacterial and fungal composition, and microbial biomass). However, the NP treatment significantly increased AOB by 64.1% and decreased the ratio of AOA/AOB by 59.6%. ③ Single addition of N and P had no significant effect on soil pH, whereas the simultaneous addition of N and P significantly reduced soil pH. Though N and P additions had no significant effect on soil base cations, structural equation models (SEM) showed that soil base cations had a direct regulatory effect on soil microorganisms (bacterial and fungal composition). ④ Soil water content had the highest explanatory power for the variation in soil microorganisms based on the results of the SEM. In conclusion, our study shows that soil AOB is more sensitive to nutrient addition, and short-term nutrient addition could increase the abundance of AOB, thus promoting nutrient transformation. © 2021, Science Press. All right reserved.

引用

页码：6058 / 6066

页数：8

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