Preparation of Lateolabrax maculatus Immune Peptides and Its Bidirectional Immunomodulatory Activity on RAW264.7 Cells

被引：0
|
作者：
Lin S. [1 ]
Chen X. [1 ]
Yang F. [1 ]
Huang M. [1 ]
Yang Q. [1 ]
Cai X. [1 ]
Wang S. [1 ]
机构：
[1] College of Biological Science and Engineering, Fuzhou University, Fuzhou
来源：
Journal of Food Science and Technology (China) | 2023年 / 41卷 / 04期
基金：
中国国家自然科学基金;
关键词：
bidirectional immunomodulatory; immune peptides; Lateolabrax maculatus immunomodulatory peptides; RAW264; 7; macrophages; response surface methodology;
D O I：
10.12301/spxb202200788
中图分类号：
学科分类号：
摘要：
To study the immune and anti-inflammatory activities of Lateolabrax maculatus immunomodulatory peptides (LIP), the preparation conditions of LIP were optimized by single factor experiments and response surface methodology with the stimulation index of mouse spleen lymphocytes as an indicator. RAW264. 7 macrophage model was used to study the immunomodulatory effect of LIP, and anti-inflammatory activity of LIP was explored by constructing a lipopolysaccharide-induced RAW264. 7 macrophage inflammatory model. The results showed that the optimal preparation conditions for LIP were as following: solid-liquid ratio 1. 0: 3. 8 (g / mL), enzyme adding amount 2. 14%, papain hydrolysis time 56 min. Under this condition, the stimulation index of mouse spleen lymphocytes reached 2. 57. The results of macrophage immunoactivity show that LIP exerted immunomodulatory activity. 400 μg / mL LIP exerted immunomodulatory effects by promoting the proliferation and phagocytosis capacity, nitric oxide and reactive oxygen species production, nitric oxide synthase (iNOS) mRNA expression, and proinflammatory cytokines secretion. Moreover, 400 g / mL LIP also showed effectively anti-inflammatory capacity by downregulating prostaglandin E2 and proinflammatory cytokines, and reducing excessive secretion of NO via inhibiting overexpression of iNOS and cyclooxygenase-2 mRNA. These results suggested that LIP exerted bidirectional immunomodulatory activity, including “increasing immune response" and “inhibiting excessive immune response ", which provided a theoretical basis for bidirectional immunomodulatory functional foods research. © 2023 Beijing Technology and Business University, Department of Science and Technology. All rights reserved.
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页码：94 / 106
页数：12
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