Synergistic Inhibition of Pseudomonas aeruginosa Biofilm by the Combination of Nisin and Honokiol

被引：0

作者：

Zhu Z. ^{[1
]}

Wang Z. ^{[1
]}

Nie R. ^{[1
]}

Liu G. ^{[1
,2
,3
]}

机构：

[1] School of Food and Health, Beijing Technology and Business University, Beijing

[2] Key Laboratory of Geriatric Nutrition and Health, Ministry of Education, Beijing Technology and Business University, Beijing

[3] Research Center of Food Additives, Beijing Technology and Business University, Beijing

来源：

Journal of Chinese Institute of Food Science and Technology | 2023年 / 23卷 / 10期

关键词：

bio film; honokiol; inhibition; Nisin; Pseudomonas aeruginosa; synergistic;

D O I：

10.16429/j.1009-7848.2023.10.002

中图分类号：

学科分类号：

摘要：

Objective: Pseudomona aeruginosa biofilm (BF) is amajor cause of persistent food contami nation. Nisin, a natural food preservative, can inhibit a variety of foodborne pathogens, but is ineffective against Gram-negative bacteria. In this study, it aimed to enhance the inhibition and BF scavenging ability of P. aeruginosa ATCC 9027 biofilm by combining Nisin with honokiol, and to investigate the related mechanism of action. Methods: Firstly, this study screened the herbal components that could inhibit P. aeruginosa BF and the minimum inhibitory concentrations; then this study evaluated the synergistic inhibitory effects of Nisin in combination with honokiol on the biofilm of P. aeruginosa, the changes of the inhibitory effects over time, and the effects on the structure, components and related gene expression of BF. Results: The strongest inhibitory effect of honokiol on the biofilm of P. aeruginosa ATCC9027 was selected as the herbal component in combination with Nisin; the MBIC50 of Nisin and honokiol were 1 mg/mL and 12.5 µg/mL, respectively, several combinations showed synergistic inhibitory effects, among which 1.5625 µ/mL of honokiol and 0.125 mg/mL Nisin had the best synergistic inhibition effect on B F, reaching the highest at 18 h. Moreover, the combination of the two significantly affected the structure of BF and reduced the content of extracellular polysaccharide and e DNA, while the expression of BF-related genes lasR, pelA, algC, pqsA and lasl was reduced. Conclusion; The combination of Nisin and honokiol phenol has a synergistic effect on the biofilm of P. aeruginosa ATCC9027, which can reduce the transcription level of BF-related genes to decrease the secretion of EPS and e DNA, thus inhibiting and scavenging BF. © 2023 Chinese Institute of Food Science and Technology. All rights reserved.

引用

页码：12 / 22

页数：10

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