Mapping a Rice Glabrous Gene Using Simple Sequence Repeat Markers

Inheritance analysis and gene mapping of the glabrous trait were conducted using the crosses between a pubescent rice material Chuanxiang 29B(an aromatic elite maintainer line of hybrid rice) ,and two glabrous rice materials,Lemont and R2(R2 is a new restorer line of hybrid rice) .All F1 plants from the two crosses had pubescent leaves,confirming that the pubescence trait in Chuanxiang 29B is dominant over the glabrous trait.In F2 individuals,the segregation ratio of three pubescent to one glabrous plant suggests that a single glabrous gene was present in Lemont and R2.Using the bulked segregant analysis,the linkage analysis of the simple sequence repeat markers showed that the glabrous gene,gl-1,was flanked by GL311 and GL8 with the genetic distances of 0.19 and 0.92 cM on chromosome 5,respectively.