DETERMINATIONS OF THE BIOCHEMISTRY FOR CYTOPLASMIC MALE-STERILITY AND THE GENETICS FOR THEIR RESTORATION IN MAIZE (Zea mays L.)

On the basis of a group of "homonucleo-hetero cytoplasmic lines" being budded, C103-cms-T, C103-cms-M, C103-cms-C, C103-cms-17A, C103-cms-19A, C103-cms-21A, C103-cms-22A and C103 (NormalCytoplasm), the biochemical analyses of anthers have been carried out, showing that mere aspartic acid andless methionine, lysine and proline are contained in CMS lines than in normal line C103 (the maintain-er) while the contents of α-amylase, esterase and peroxidase isozymes in CMS lines are significantly dif-ferent from those in their maintainer. The genetic determination for restoration of the CMS lines indi-cates that types T, S(M), C, 21A can be distinguished from each other by using normal lines N6, 74-21, 525 as tester in subsequent test-cross. In addition, the correspondence relationship between the dataof biochemical and genetic determinations has also been observed. Therefore, the method of biochemicalanalysis could be used as a supplementary approach to the conventional test-cross method.