Effects of Ginsenoside Rg-1 on the Proliferation and Osteogenic Differentiation of Human Periodontal Ligament Stem Cells

Objective:To evaluate the effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells(hPDLSCs)and to explore the possible application on the alveolar bone regeneration.Methods:To determine the optimum concentration,the effects of ginsenoside Rg-1ranging from 10 to 100μmol/L were evaluated by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide,alkaline phosphatase activity and calcium deposition.Expressions of runt-related transcription factor 2,collagen alpha-2(l)chain,osteopontin,osteocalcin protein were examined using real-time polymerase chain reaction.Results:Compared with the control group,a certain concentration(10μmol/L)of the Rg-1 solution significantly enhanced the proliferation and osteogenic differentiation of hPDLSCs(P<0.05).However,concentrations that exceeds 100μmol/L led to cytotoxicity whereas concentrations below 10 nmol/L showed no significant effect as compared with the control.Conclusion:Ginsenoside Rg-1 can enhance the proliferation and osteogenic differentiation of hPDLSCs at an optimal concentration of 10μmol/L.