Effect of Chinese Drugs for Activating Blood Circulation and Detoxifying on Indices of Thrombosis,Inflammatory Reaction,and Tissue Damage in A Rabbit Model of Toxin-Heat and Blood Stasis Syndrome

<正>Objective:To observe the regulatory effect of Chinese drugs for activating blood circulation(ABC) and for activating blood circulation and detoxifying(ABCD) on indices of thrombosis,inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome.Methods:Fifty-four rabbits were randomized into the normal control group,model group,simvastatin group(simvastatin,0.93 mg/kg per day),ABC group[Xiongshao Capsule(芎芍胶囊),0.07 g/kg per day],and ABCD group[Xiongshao Capsule,0.07 g/kg per day,and Huanglian Capsule(黄军胶囊),0.14 g/kg per day].All except the normal control group received a single injection of bovine serum albumin and were fed with high-fat diets for 6 weeks.At the end of week 4 of giving highfat diets,a dose of endoxitin was given by ear vein injection,and a randomized 2-week treatment was initiated. At the end of treatment,blood lipids,circulating endothelial cells,and the pathological changes of the aortic arch were assessed.The serum levels of matrix met alloproteinases(MMP-9),tissue inhibitors to met alloproteinase (TIMP-1),granule membrane protein-140(GMP-140),plasminogen activator inhibitor-1(PAI-1),high-sensitivity C-reactive protein(hs-CRP),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α) were determined.Results: Compared with the model group,ABCD group showed decreased serum triglyceride(TG) level,improvement in the pathological change in the aortic arch,and reduction in the number of circulating endothelial cells(4.00±1.41 per 0.9μL for ABCD group vs 7.83±1.72 per 0.9μl L for the model group).In addition,the levels of serum GMP-140,PAI-1,and IL-6 in ABCD group were also significantly reduced[0.79±0.20 ng/mL,5.23±1.39 ng/mL, 40.64±10.11 pg/mL for ABCD group vs 1.08±0.31 ng/mL,7.28±2.01 ng/mL,54.44±13.56 pg/mL for the model group,respectively,P<0.05].A trend showing improvement in the indices of thrombosis,inflammatory reaction, and tissue damage was observed in the ABC group when compared to the model group,but the changes were not statistically significant(P>0.05).Conclusions:Chinese drugs for activating blood circulation and detoxifying have beneficial effects on regulating indices of thrombosis(GMP-140 and PAI-1) and inflammatory reaction(IL-6) in rabbit model with toxic-heat and blood stasis.The effect of the activating blood circulation and detoxifying drugs in regulating the levels of serum GMP-140,PAI-1,and IL-6 was superior to that of the activating blood circulation drugs.